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Purification preparation method and application of mesenchymal stem cell secreted factors

A technique of secreting factors and mesenchymal stem cells, which is applied in the field of skin repair, can solve problems such as ineffective removal, and achieve the effect of improving complexion, eliminating skin wrinkles, and shrinking pores

Active Publication Date: 2021-09-24
铜仁市泛特尔生物技术有限公司
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Problems solved by technology

[0004] At present, there are also some methods for preparing the supernatant of mesenchymal stem cells. A typical case is to concentrate the collected supernatant by ultrafiltration (for example, 3kD), and then add certain substances to make a preparation or freeze-dry; but it cannot Effectively remove some macromolecular substances in the medium, such as exogenous proteins, sugars, and impurities in the medium that cannot pass through the 3kD ultrafiltration membrane

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  • Purification preparation method and application of mesenchymal stem cell secreted factors
  • Purification preparation method and application of mesenchymal stem cell secreted factors
  • Purification preparation method and application of mesenchymal stem cell secreted factors

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Embodiment Construction

[0037] In order to make the realization technical means, creative features, purpose and effect of the present invention easy to understand, the present invention is further elaborated below.

[0038] 1. Preparation method

[0039] Such as figure 1 , figure 2 As shown, a method for purifying, preparing and using a mesenchymal stem cell secreted factor, comprising the following steps:

[0040] The source of the umbilical cord of the present invention: Parents of the fetus sign the informed consent form of the umbilical cord donor, check the maternal hepatitis C virus antibody, HIV I / II antibody, hepatitis B virus antigen, Treponema pallidum antibody, and hepatitis A virus antibody before delivery and cytomegalovirus IgM antibodies. There is an infectious disease screening before the collection, and the time is not limited; there is another screening after the collection, with an interval of 7 weeks between the two screenings, and the above infectious disease inspection resul...

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Abstract

The invention provides a purification preparation method and an application of mesenchymal stem cell secreted factors. The purification preparation method comprises the following steps: (1) preparing MSC supernate; (2), purifying the mesenchymal stem cell secreted factors, including filtration and concentration, heparin affinity chromatography, and heparin affinity chromatography elution peak 5kD ultrafiltration or G-25 desalination column desalination; and (3) using the purified MSC secretion factors in the step (3) to prepare masks. According to the invention, a heparin affinity chromatography medium is adopted to specifically adsorb mesenchymal stem cell (MSC) secreted factors and purify substances with an effect of stimulating NIH 3T3 cell proliferation in MSC secretions, and the substances are potentially used for repairing refractory wounds and are added to cosmetics as an additive. The effects of repairing damaged skin, eliminating skin wrinkles, shrinking pores, improving complexion and the like can be achieved.

Description

technical field [0001] The invention belongs to the technical field of skin restoration, and specifically relates to a purification preparation method and application of mesenchymal stem cell secreted factors. Background technique [0002] Human mesenchymal stem cells (MSCs) are a type of fibroblast-like morphology (spindle and spindle) after adherent culture, which can self-renew in vitro and have osteogenic, adipogenic, and chondrogenic differentiation. capable stem cells. It can not only differentiate into various human cells, including adipocytes, osteoblasts, chondrocytes, muscle cells, tenocytes, etc.; at the same time, MSCs can also be induced to differentiate into liver cells, pancreatic beta cells, nerve cells, etc. Human mesenchymal stem cells can be isolated from a variety of human tissues (such as bone marrow, umbilical cord, placenta, fat, umbilical cord blood, etc.), and can also be obtained by differentiation or transdifferentiation; mesenchymal stem cells fr...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/9789A61K8/9794A61K8/9706A61K8/19A61K8/34A61K8/65A61K8/67A61K8/68A61K8/73A61P17/00A61Q19/00C07K1/22C07K1/34C07K1/36C12P21/00
CPCA61K8/64A61K8/9789A61K8/645A61K8/9794A61K8/65A61K8/735A61K8/99A61K8/9706A61K8/675A61K8/19A61K8/68A61K8/345A61Q19/00A61P17/00C12P21/00C07K1/34C07K1/22C07K1/36A61K2800/85Y02A50/30
Inventor 邹邦银敖维飞熊尚琦
Owner 铜仁市泛特尔生物技术有限公司
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