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Thin-layer chromatography detection method for exogenous mixture II

A technology of thin-layer chromatography and detection methods, applied in the direction of measuring devices, analysis materials, instruments, etc.

Pending Publication Date: 2021-09-21
南宁市第一人民医院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no quality control standard for Waigan No. 2 mixture, so we established a TLC identification method for Zhonggangmei in Waigan No. 2 mixture

Method used

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  • Thin-layer chromatography detection method for exogenous mixture II

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Embodiment Construction

[0015] The present invention will be further illustrated below in conjunction with the examples, which are only used to explain the present invention, but do not limit the scope of the present invention.

[0016] Use the TLC method to carry out qualitative identification of Waigan No. 2 mixture according to the following steps:

[0017] 1. Instruments and materials

[0018] 1.1 Instruments and reagents

[0019] Instruments: constant temperature and humidity chamber (model HO250HSX, Shanghai Suying Test Instrument Co., Ltd.); numerical control ultrasonic cleaner (model KQ-100DE, Kunshan Ultrasonic Instrument Co., Ltd.); digital constant temperature water bath (model HH-6 , Changzhou Guohua Electric Appliance Co., Ltd.); one millionth electronic analytical balance (model KY-083, Guangdong Xindongyuan Life Science and Technology Development Co., Ltd.); silica gel G thin-layer board (batch number 20181208, specification 10×20cm, Qingdao Ocean Chemical Branch).

[0020] Reagents...

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Abstract

The invention discloses a technology for qualitatively identifying an exogenous mixture II (which is prepared by extracting medicinal materials such as schefflera octophylla bark, evodia lepta, roughhaired holly and helicteres angustifolia through a scientific method) by adopting a thin-layer chromatography identification method. The method belongs to the technical field of medicines and comprises the steps of preparation of a reference solution, preparation of a test solution, chromatographic conditions and the like. According to the method disclosed by the invention, qualitative identification is carried out on the exogenous mixture II by adopting the thin-layer chromatography identification method and material established by the invention, and the method is high in specificity and good in stability and can be used for quality control of an exogenous mixture II product.

Description

technical field [0001] The invention belongs to a quality control method of Waigan No. 2 mixture (which is prepared by scientifically extracting and preparing medical materials such as duck foot veneer, trichaku, ganglion and mountain sesame). Background technique [0002] Influenza is a clinically common disease. Traditional Chinese medicine, as a traditional medical technology in my country, has conducted in-depth research on the syndrome differentiation and treatment of colds since ancient times. According to the theoretical analysis of traditional Chinese medicine, a cold is caused by the invasion of the human body by the six evils and viruses. Among them, wind evil is the first of the six evils, which is the main cause of colds. Because there are different levels of pathogenicity, different strengths of righteousness, and differences in the four seasons and six qi, the symptoms are also slightly different. According to the difference of pulse syndrome and tongue synd...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/94G01N30/95
CPCG01N30/90G01N30/94G01N30/95
Inventor 秦艳娥蓝晓步黄兴振许崇摇植国繁董思喆
Owner 南宁市第一人民医院
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