Creatinine quantitative rapid detection test strip and preparation method thereof

Abstract

The invention discloses a creatinine quantitative rapid detection test strip, which comprises a bottom plate, and a sample pad, an enzyme working pad, an NC film and a PC film which are sequentially arranged on the bottom plate from one end to the other end, the other end of the sample pad is lapped at one end of the enzyme working pad, and the other end of the enzyme working pad is lapped at one end of the NC film; one end, far away from the NC film, of the PC film is supported, so that the other end of the PC film is partially suspended above the other end of the NC film, and a color developing agent is uniformly coated on one surface, close to the NC film, of the PC film and an area in contact with the NC film after the PC film is pressed down. The invention also discloses a preparation method of the test strip for quantitative and rapid detection of creatinine and a kit. According to the invention, the lateral chromatography technology and the design of separating the reaction area from the sample adding area are adopted, and the color development is rapid and uniform through a pressing contact color development mode.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a creatinine quantitative rapid detection test strip and a preparation method thereof. Background technique [0002] With the progress of research on the mechanism of diabetic nephropathy, early indicators for predicting diabetic nephropathy have gradually been discovered and applied clinically, but the current gold standard for diagnosing diabetic nephropathy is to measure the excretion rate of microalbumin in urine in 24 hours or overnight. However, it is cumbersome, time-consuming, and poor patient compliance to collect 24-hour urine or overnight urine samples. Therefore, the clinical application of both is limited. Studies have shown that urine protein / urine creatinine ratio (urinary albumin-to-creatinineratio, ACR) measurement is a sensitive indicator of early kidney damage in diabetes, and can be used to monitor urinary protein excretion, using urinary creatinine...

AI Technical Summary

Problems solved by technology

Therefore, because the sample loading area and the color development area are at the same position, the color development is easily interfered by the sample; the reagent layer and the indicator layer are in contact, and the indicator layer is easy to change color, etc.

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