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Sea squirt antioxidant polypeptide and preparation method thereof

A technology of antioxidant peptides and sea squirts, applied in the biological field, can solve the problems of less research on antioxidant active peptides, and achieve the effect of increasing added value and broadening research

Active Publication Date: 2021-09-21
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protein content in true sea squirt accounts for 34%, which is the substance with the most content except water, but there are few studies on the antioxidant active peptides in true sea squirt

Method used

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  • Sea squirt antioxidant polypeptide and preparation method thereof
  • Sea squirt antioxidant polypeptide and preparation method thereof
  • Sea squirt antioxidant polypeptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation of sea squirt antioxidant polypeptide comprises the following steps:

[0035] 1) Treatment of true sea squirts: after thawing, remove the outer shell of true sea squirts to take the inner capsule, remove the viscera and fascia in the inner capsule, add ultrapure water to boil at 100°C for 15 minutes to inactivate enzymes, and prepare meat pieces of the sea squirt inner capsule for use.

[0036] 2) Impurity removal: After the meat pieces are crushed, they are placed in petroleum ether to remove fat, and ethanol is added to remove pigment to obtain ascidian crude protein, which is ready for use.

[0037] 3) Enzymatic hydrolysis of ascidian internal capsule protein: use complex protease to enzymolyze the internal capsule protein of sea squirt, pH is 7.0, keep the temperature in a water bath at 50°C, the ratio of enzyme to substrate is 1:4, enzymatic hydrolysis for 7 hours, then boil in water bath for 15 minutes Inactivate the enzyme, quickly cool to room te...

Embodiment 2

[0049] Example 2 Determination of antioxidant activity

[0050] 1) DPPH free radical scavenging ability

[0051] 95% ethanol prepared DPPH radical solution with a concentration of 0.1mM. Samples with different concentrations were mixed with DPPH at a ratio of 1:1, and the absorbance was measured at 517nm after reacting at room temperature for 30 minutes in the dark. The blank group replaced the sample with the same volume of 95% ethanol, and the DPPH free radical scavenging ability of the sample was calculated according to the following formula:

[0052]

[0053] In the formula, A control Absorbance value of the blank group, A sample is the absorbance value of the experimental group.

[0054] 2) ABTS free radical scavenging ability

[0055] ABTS solution with a concentration of 7mM and potassium persulfate solution with a concentration of 2.45mM were prepared in ultrapure water, mixed at a ratio of 1:1, and incubated at room temperature in the dark for 12-16 hours to ob...

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Abstract

The invention discloses a sea squirt antioxidant polypeptide and a preparation method thereof. The amino acid sequence of the antioxidant polypeptide is Ile-Ser-Trp. The preparation method comprises the following steps: carrying out enzymolysis on sea squirt inner capsule meat blocks serving as a raw material by using compound protease, separating, purifying, and freeze-drying to obtain the antioxidant polypeptide. The antioxidant polypeptide relieves side effects possibly caused by artificial antioxidants, provides a new natural antioxidant, and can replace traditional synthetic food antioxidants.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a sea squirt antioxidant polypeptide and a preparation method thereof. Background technique [0002] The human body will produce a large number of free radicals every day, and environmental factors will also promote the production of free radicals in the human body. However, it will oxidize the normal substances of the human body, destroy the cell structure, promote the malignant transformation of cells, and cause damage to the body cells and tissues. The negative effects of free radicals are related to the aging of the human body and many health diseases, such as diabetes, cancer, and neurodegenerative diseases. [0003] There are some natural antioxidants in the human body that can eliminate the harm of free radicals to the human body, such as vitamin C and vitamin E, and some synthetic antioxidants can also eliminate free radicals. However, studies have shown that syn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/083C07K1/36C07K1/34C07K1/16C12P21/06
CPCC07K5/0808C12P21/06
Inventor 卜迁麻蕊侯青高鸿
Owner SICHUAN UNIV
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