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Compound traditional Chinese medicine composition for inhibiting cow-derived salmonella and application

A compound traditional Chinese medicine and Salmonella technology, applied in antibacterial drugs, medical raw materials derived from arthropods, pharmaceutical formulations, etc., can solve problems such as complex principles and different mechanisms of action, and achieve less drug residues, less toxic and side effects, and lower GOT and the effect of GPT levels

Inactive Publication Date: 2021-09-17
HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many herbal medicines have antibacterial and antiviral effects, but the mechanism of action is different from that of western medicine. Among them, the antibacterial mechanism of compound Chinese herbal medicine is not yet clear, and the principle is relatively complicated

Method used

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  • Compound traditional Chinese medicine composition for inhibiting cow-derived salmonella and application
  • Compound traditional Chinese medicine composition for inhibiting cow-derived salmonella and application
  • Compound traditional Chinese medicine composition for inhibiting cow-derived salmonella and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Experimental Animals and Strains

[0026] Sixty SPF grade BALB / C mice, about 19 days old, with a body weight of 18-20 g.

[0027] The median lethal dose (LD) of strain HB1 to 19-day-old mice of 7 strains of Salmonella from dairy cows numbered HB1~HB7 (serotype: HB1~HB6 is Salmonella paratyphi A, HB7 is Salmonella paratyphi B). 50 ) is 1×10 7 CFU / mL, the median lethal dose (LD) of strain HB7 to 19-day-old mice 50 ) is 5.6×10 6 CFU / mL, isolated, identified and preserved by Hebei Provincial Key Laboratory of Preventive Veterinary Medicine.

[0028] Preparation and counting of bacterial liquid

[0029] Take out the preserved Salmonella from the -80°C refrigerator and streak in SS medium for recovery, pick a single colony and culture it in LB solution at 37°C to cause logarithmic growth phase, and adjust the bacterial count of strains HB1 and HB7 by counting plate counting method The liquid concentration is 1×10 7 CFU / mL and 5.6×10 6 CFU / mL.

Embodiment 2

[0031] Preparation of traditional Chinese medicine composition

[0032] A compound traditional Chinese medicine composition for inhibiting cow-derived Salmonella, comprising the following raw materials: ebony plum, gallnut, schisandra, scutellaria baicalensis, and licorice.

[0033] In order to further optimize the technical scheme: the mass ratio of ebony, Chinese gall, schisandra, scutellaria baicalensis, and licorice is 1:5:1:2:1.

[0034] The preparation method comprises the following steps:

[0035] 1) Weigh each raw material and mix, add distilled water, and soak;

[0036] 2) After boiling with a strong fire, simmer, filter, and collect the filtrate; repeat 2 times, combine the 2 filtrates and concentrate to obtain a concentrated solution;

[0037] 3) Centrifuge the concentrated solution, take the supernatant and sterilize it, and obtain a compound traditional Chinese medicine composition for inhibiting salmonella from cows.

[0038] For further optimizing the technic...

Embodiment 3

[0043] Preparation of traditional Chinese medicine composition

[0044] A compound traditional Chinese medicine composition for inhibiting cow-derived Salmonella, comprising the following raw materials: ebony plum, gallnut, schisandra, scutellaria baicalensis, and licorice.

[0045] In order to further optimize the technical scheme: the mass ratio of ebony, Chinese gall, schisandra, scutellaria baicalensis, and licorice is 1:1:1:1:1.

[0046] The preparation method comprises the following steps:

[0047] 1) Weigh each raw material and mix, add distilled water, and soak;

[0048] 2) After boiling with a strong fire, simmer, filter, and collect the filtrate; repeat 2 times, combine the 2 filtrates and concentrate to obtain a concentrated solution;

[0049] 3) Centrifuge the concentrated solution, take the supernatant and sterilize it, and obtain a compound traditional Chinese medicine composition for inhibiting salmonella from cows.

[0050] For further optimizing the technic...

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Abstract

The invention discloses a compound traditional Chinese medicine composition for inhibiting cow-derived salmonella and application, and relates to the technical field of traditional Chinese medicine compositions. The compound traditional Chinese medicine composition comprises the following raw materials: dark plum, gallnut, schisandra chinensis, scutellaria baicalensis and liquorice. The invention also provides a preparation method and application. The compound traditional Chinese medicine composition has the advantages that the average antibacterial diameter of the compound traditional Chinese medicine composition to the cow-derived salmonella is 26.7 mm, and the average MIC and MBC are 0.56 mg / ml and 1.12 mg / ml respectively, so that the compound traditional Chinese medicine composition is good in in-vitro antibacterial effect; and the compound traditional Chinese medicine composition can improve the TP and IgG contents and reduce the GOT and GPT levels in mouse serum, and has a certain effect on prevention and treatment of cow-derived salmonella infection.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a compound traditional Chinese medicine composition for inhibiting cow-derived Salmonella and its application. Background technique [0002] The main clinical symptoms of salmonellosis in dairy cows are septicemia, high fever, and diarrhea. This disease can occur all year round and seriously affects the development of the breeding industry. At present, antibiotics are mainly used to prevent and control salmonellosis in dairy cows. However, due to the abuse of antibiotics, multiple The emergence of drug-resistant Salmonella has greatly affected the therapeutic effect of antibiotics. [0003] Chinese herbal medicine is the product of continuous practice and experimentation for thousands of years, and it is a treasure of Chinese traditional culture. Many herbal medicines have antibacterial and antiviral effects, but the mechanism of action is diffe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/736A61K36/79A61P31/04A61K35/64
CPCA61K36/736A61K35/64A61K36/79A61K36/57A61K36/539A61K36/484A61P31/04A61K2236/331A61K2236/39A61K2236/51A61K2236/53A61K2300/00
Inventor 张艳英郭家媚赵允清邵明珠王璐李涵吴同垒张志强
Owner HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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