Fluorescence detection system and method based on double-core photonic crystal fiber

Abstract

The invention relates to a fluorescence detection system and method based on a double-core photonic crystal fiber. A to-be-detected fluorescence sample solution is poured into an air hole of the double-core photonic crystal fiber through a liquid absorption device; one end of the double-core photonic crystal fiber is connected to a spectrometer module, and the other end of the double-core photonic crystal fiber is connected to a laser through a single-mode fiber; a first fiber core in the double-core photonic crystal fiber outputs excitation laser; a second fiber core in the double-core photonic crystal fiber outputs a fluorescence signal; and the fluorescence signal is coupled to a spectrograph module for subsequent analysis. Background laser can be effectively filtered, the micro-fluidic function and the fluorescence excitation enhancing function are achieved, and a good detection effect can be obtained under the condition that only a small number of samples are consumed.

Description

technical field [0001] The present invention relates to fluorescence detection, in particular to a fluorescence detection system and method based on a dual-core photonic crystal fiber. A liquid suction device is used to pour a fluorescent sample solution to be measured into the air hole of a double-core photonic crystal fiber, and the dual-core photonic crystal fiber One end of the core photonic crystal fiber is connected to the spectrometer module, the other end of the dual-core photonic crystal fiber is connected to the laser through a single-mode fiber, the first core in the dual-core photonic crystal fiber outputs excitation laser light, and the dual-core photonic crystal fiber The second fiber core in the core photonic crystal fiber outputs a fluorescent signal, which is coupled to the spectrometer module for subsequent analysis, which can effectively filter the background laser light, and has the functions of microfluidic control and enhanced fluorescence excitation, whic...

AI Technical Summary

Problems solved by technology

However, such a fluorescence detection system has certain limitations: (1) it is difficult to achieve miniaturization and integration; (2) due to the application of space optics, certain requirements are placed on the stability of the system; (3) due to Different devices are introduced for fluorescence processing, which reduces the reliability of the system; (4) a relatively large number of samples are required to obtain better detection results

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