Multiplex fluorescent quantitative RT-PCR (reverse transcription-polymerase chain reaction) kit for detecting hematogenous infectious viruses

Abstract

The invention provides a multiplex fluorescent quantitative RT-PCR (reverse transcription-polymerase chain reaction) kit for detecting hematogenous infectious viruses. The multiplex fluorescent quantitative RT-PCR kit comprises quantitative RT-PCR reaction liquid, enzyme Mix liquid, primer probe Mix liquid, standard substances (cytomegalovirus, Epstein-Barr virus, herpes simplex virus and RNase P standard substances), positive reference substances and negative reference substances. The primer probes comprise four groups of primers and corresponding probes with different fluorescence labels. According to the kit, high-specificity primer probes are designed according to high-conservative proteins of cytomegalovirus, Epstein-Barr virus, herpes simplex virus and RNase P correspondingly, and whether the viruses exist in a serum sample or not is simultaneously detected through a PCR reaction. The kit disclosed by the invention is simple, convenient and rapid, cost-saving, good in repeatability and capable of effectively and rapidly screening the hematogenous infectious viruses. The kit can be applied to rapid screening of the hematogenous infectious viruses and research of hematogenous infectious virus epidemiology.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a fluorescent quantitative RT-PCR detection kit, in particular to a multiple fluorescent quantitative RT-PCR kit for detecting blood-borne infectious viruses, which is a one-step method for multiple real-time fluorescent quantitative RT-PCR at the same time. A nucleic acid detection method for detecting cytomegalovirus (CMV), Epstein-Barr virus (EBV), and herpes simplex virus (HSV) in serum samples in a reaction tube, which can be applied to rapid screening of blood-borne infectious viruses and blood-borne A study of the epidemiology of infectious viruses. Background technique [0002] In the past few decades, people have become more and more aware of the serious threat of infectious pathogens to blood safety. Blood-borne infectious pathogens are mainly transmitted through blood donated by pathogen-carrying blood donors. Therefore, the screening strategy for blood-borne infect...

AI Technical Summary

Problems solved by technology

Although the screening of the four pathogens can effectively guarantee the safety of clinical blood use in my country and most countries and regions in the world, as the prevalence of new (emergency) pathogens and undetected pathogens increases significantly, international cooperation and exchanges It has become more and more frequent. In recent years, imported blood-borne infectious pathogens have occurred continuously, which increases the risk of pathogen transmission and seriously threatens the safe use of blood in clinical practice. The project is imminent

Nucleic acid detection technology is direct and has high specificity and sensitivity for detecting trace infectious pathogens. However, factors such as expensive detection costs and long operation time seriously affect the wide application of this technology.

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