Stem cell-derived exosome composition for epidermal repair, and preparation method and application thereof

An epidermal repair and exosome technology, applied in skin care preparations, animal cells, cosmetic preparations, etc., can solve the problems of poor skin epidermal repair effect, inability to use skin care products, tingling and other problems

Pending Publication Date: 2021-09-10
汉氏盛弘生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the continuous development of the economy and the continuous change of the environment, people's skin is also vulnerable to various aspects and causes various problems, such as allergies, redness, dry itching, acne, stinging and other problems, and it is impossible to use any skin care products. Seriously affect people's life and work
However, there is currently no skin preparation that works well for recurring skin problems such as allergies, redness, acne, dry itching and stinging
The current skin preparations, such as various skin care products, mostly stay in the stratum corneum, and the repair effect on the problem skin epidermis is not good, and cannot meet people's increasingly urgent skin needs

Method used

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  • Stem cell-derived exosome composition for epidermal repair, and preparation method and application thereof
  • Stem cell-derived exosome composition for epidermal repair, and preparation method and application thereof

Examples

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preparation example Construction

[0024] On the other hand, the embodiment of the present application provides a method for preparing a stem cell-derived exosome composition for epidermis repair, comprising the following steps: dissolving rehmannia glutinosa extract, Chuanxiong extract, and Radix Radix Radix Rhizoma Rhizome in a part of the solvent, Mix to obtain mixed solution 1, then add mixed solution 1 and functional additives to the remaining solvent to obtain mixture 2, then add composite gel freeze-dried powder and human adipose-derived mesenchymal stem cell freeze-dried powder at 4°C and human adipose stem cell-derived exosomes, and homogenize to obtain the stem cell-derived exosome composition for epidermal repair.

[0025] In another aspect, the embodiment of the present application provides an application of the aforementioned stem cell-derived exosome composition for epidermal repair in the preparation of skin care products and / or cosmetics.

Embodiment 1

[0028] The purpose of this example is to provide exosomes derived from human adipose-derived mesenchymal stem cells.

[0029] 1. Culture human adipose-derived mesenchymal stem cells

[0030] Collect 10 mL of fat from normal people, wash it three times with PBS to remove stains, cut it into pieces, and digest it with 0.12% type I collagenase at a temperature of 37°C for 30 minutes. After digestion, add 2 mL of 10% fetal bovine serum to stop Digest, centrifuge at 1500g for 10min to get the precipitate, resuspend the precipitate and pass through a 200-mesh cell sieve twice, collect the filtrate and centrifuge to get the precipitate, resuspend the precipitate to 5×10 4 / mL, inoculated in 6-well plate, placed at 37°C, 5% CO 2 Cultivate in an incubator, change the medium for the first time after the cells are completely attached to the wall, change the complete medium every 2 days after the first medium change, and add 1ml of digestive solution (0.25% Trypsin-0.53mM EDTA) for dige...

Embodiment 2

[0040] The purpose of this example is to provide a stem cell-derived exosome composition for epidermal repair, which is mainly prepared through the following steps:

[0041] 1. Preparation of composite gel

[0042] Collagen is made into a 1.2wt% collagen solution, sodium hyaluronate is made into a 1.0% sodium hyaluronate solution, the collagen solution and sodium hyaluronate solution are mixed, and a final concentration of 0.02mol / L genipin was cross-linked at 45°C for 2.5h to obtain a mixture, and the mixture was pre-frozen and then freeze-dried at -80°C for 48h to obtain the composite gel lyophilized powder.

[0043] 2. Preparation of exosomes

[0044] Exosomes were prepared according to the steps in Example 1, and exosomes with a purity of more than 96% and a concentration of 2.0 g / L were selected.

[0045] 3. Preparation of plant extract working solution

[0046] Rehmannia glutinosa extract, Chuanxiong extract and Fangfeng root extract were dissolved in water respectively...

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Abstract

The invention provides a stem cell-derived exosome composition for epidermal repair, and a preparation method and application thereof, and relates to the technical field of biological materials. The compositio comprises a human adipose-derived stem cell-derived exosome. A preparation method of the composition and an application of the composition in skin care products and / or cosmetics are further provided. The composition has the advantages that the composition can effectively repair skin epidermis, and inhibit recurrence of epidermis damage, such as allergy, redness, dry itching, acne and stabbing pain.

Description

technical field [0001] The present invention relates to the technical field of biomaterials, in particular, to a stem cell-derived exosome composition, preparation method and application for epidermal repair. Background technique [0002] People's skin is composed of epidermis, dermis and subcutaneous tissue, and contains accessory organs (sweat glands, sebaceous glands, fingernails and toenails), blood vessels, lymphatic vessels, nerves and muscles. The epidermis is the outermost layer of the skin and the first barrier of the human body. It resists various harmful factors from the outside world. The average thickness is 0.2 mm. According to the different development stages and morphological characteristics of cells, it can be divided into 5 layers from the outside to the inside. Including stratum corneum, clear layer, granular layer, acanthocyte layer and basal layer. [0003] With the continuous development of the economy and the continuous change of the environment, peop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/65A61K8/73A61K8/9789A61Q19/00C12N5/0775
CPCA61K8/99A61K8/735A61K8/65A61K8/9789A61Q19/00C12N5/0667A61K2800/5922A61K2800/84
Inventor 任雪涛
Owner 汉氏盛弘生物科技有限公司
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