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Application of compound LM22B-10 in preparation of medicine for treating corneal epithelium and nerve injury

A technology of LM22B-10 and nerve injury, applied in the field of biomedicine, can solve the lack of evaluation of corneal nerve injury repair and other problems, achieve important development and application prospects, reduce eye symptoms, and reduce drug toxicity

Active Publication Date: 2021-09-07
AIER EYE HOSPITAL GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Also missing from these techniques is the assessment of corneal nerve injury repair in diabetic disease models

Method used

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  • Application of compound LM22B-10 in preparation of medicine for treating corneal epithelium and nerve injury
  • Application of compound LM22B-10 in preparation of medicine for treating corneal epithelium and nerve injury
  • Application of compound LM22B-10 in preparation of medicine for treating corneal epithelium and nerve injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1, compound LM22B-10 promotes the growth of nerve fibers of dorsal root ganglia in an environment without the presence of neurotrophic factors

[0039] 1. Subjects

[0040] BWEL chicken fertilized eggs were incubated at 37.8°C for 7-10 days, and the dorsal root ganglion was extracted and cultured in vitro.

[0041] 2. Experimental Drugs

[0042]LM22B-10 was dissolved in DMSO (dimethyl sulfoxide) and prepared as a stock solution at a concentration of 10 mM. The mother solution was diluted into DMEM / F12 medium containing 5% FBS (fetal bovine serum), and the concentrations were 1, 10, 100, 1000, 10000 nM, respectively.

[0043] 3. Experimental method

[0044] After cultured for 1 day, dorsal root ganglia of chicken embryos were added with different concentration gradients of LM22B-10 medium, and after cultured for 3 days, immunofluorescence staining was performed. Neurons were stained with βIII-tubulin and then imaged for quantification of nerve fiber length. ...

Embodiment 2

[0050] Example 2, Compound LM22B-10 Accelerates Repair of Corneal Nerve Damage in Normal Mice and Type I Diabetic Mice

[0051] 1. Subjects

[0052] C57BL / 6 mice meet the age of 6-8 weeks and weigh 15-20g. The eyes of the animals were examined within 24 hours before the experiment, and animals without symptoms of eye irritation, corneal defects, or conjunctival damage were used for the experiment.

[0053] 2. Experimental Drugs

[0054] LM22B-10 was dissolved in DMSO and prepared as a stock solution at a concentration of 10 mM. The stock solution was diluted into sterile PBS (phosphate buffered saline) to prepare eye drops at a concentration of 1 μM. The control group was PBS containing the same amount of DMSO.

[0055] 3. Establishment of mouse corneal nerve injury animal model and eye drop administration

[0056] C57BL / 6 mice were intraperitoneally injected with STZ-citrate solution for 5 consecutive days according to the STZ (streptozotocin) 50 mg / kg standard. The sta...

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Abstract

The invention relates to the field of biological medicine, in particular to application of a compound LM22B-10 in preparation of a medicine for repairing corneal epithelium and nerve trauma. The invention discloses application of the compound LM22B-10 in preparation of a medicine for treating corneal epithelium and nerve injury, and belongs to the field of biological medicine. Pharmacodynamic tests prove that after normal mice with corneal epithelium injury and type I diabetic mice are taken as experimental subjects and LM22B-10 eye drop administration is carried out, the length and density of corneal nerve fibers are remarkably increased, the corneal epithelium recovery speed is obviously increased, and the corneal sensitivity is basically recovered to be normal. The compound has a relatively good treatment effect on corneal nerve injury caused by keratitis, xerophthalmia, full femtosecond laser myopia correction, excimer laser surgery, diabetes and the like, and eye symptoms of patients can be relieved; and the drug toxicity is small, the stability is good, and important development and application prospects are realized.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of compound LM22B-10 in the preparation of medicaments for repairing corneal epithelium and nerve trauma. Background technique [0002] The cornea is the transparent tissue at the front of the eye that covers the iris, pupil, and anterior chamber of the eye. In the eyeball, the cornea, anterior chamber and lens play the role of refraction, among which the cornea has the strongest refraction ability. In the human eye, the cornea accounts for three-quarters of the total refraction. Another major function of the cornea is transparency. Only by maintaining a high degree of transparency can light be put into the eyeball. The cornea is avascular due to its transparency, and oxygen and other nutrients are passed through tears and aqueous humor to maintain the health of the cornea. The human cornea is in contact with the sclera through the limbus, and together the cornea an...

Claims

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Application Information

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IPC IPC(8): A61K31/136A61P27/02A61P25/00
CPCA61K31/136A61P27/02A61P25/00
Inventor 崔泽凯陈建苏唐仕波
Owner AIER EYE HOSPITAL GRP CO LTD
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