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Application of small molecule based on indole tricarbocyanine structure in preparation of tumor photothermal therapy drug

A technology of indole tricarbocyanine and photothermal therapy, which is applied in the field of medicine, can solve the problems that the application has not yet been discovered and reported, and achieve the effects of avoiding high biological toxicity, high photothermal conversion rate, and good water solubility

Active Publication Date: 2021-09-03
NANJING NUOYUAN MEDICAL DEVICES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention finds that the compound is an excellent drug for photothermal therapy of tumors, but this application has not been discovered and reported yet

Method used

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  • Application of small molecule based on indole tricarbocyanine structure in preparation of tumor photothermal therapy drug
  • Application of small molecule based on indole tricarbocyanine structure in preparation of tumor photothermal therapy drug
  • Application of small molecule based on indole tricarbocyanine structure in preparation of tumor photothermal therapy drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 1. Prepare an ICG-II solution with a concentration of 10 μM, and measure its temperature change under different powers;

[0064] Weigh a certain amount of ICG-II, dissolve it in DMSO, configure the mother solution with a concentration of 1 mM, then dilute the mother solution with deionized water, and configure the experimental group solution with a concentration of 10 μM. Using a 785 nm laser, the Its power density is adjusted to 1.0 W / cm respectively 2 , 1.5 W / cm 2 , 2.0 W / cm 2 , 2.5 W / cm 2 , under light conditions, use a thermocouple thermometer to measure the temperature with a time interval of 30 s. After the temperature reached the maximum temperature and stabilized, the laser was turned off and its cooling time was measured.

[0065] 2. Configure ICG-II with different concentrations, and measure ICG-II with different concentrations at 2.5 W / cm 2 lower temperature changes;

[0066] Weigh a certain amount of ICG-II, dissolve it in DMSO, and prepare a mother so...

Embodiment 2

[0096] 1 . Using HepG2 cancer cells and L02 normal cells to study the biological toxicity of the tumor treatment drug ICG-II under non-light conditions through MTT experiments.

[0097] HepG2 cell line and L02 cell line were selected as experimental cells, and MTT analysis was performed according to the standard protocol (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), To verify the biocompatibility of the probe. Add cells grown to the appropriate growth phase to 96-well cell culture plates and incubate for 24 hours under standard cell culture conditions. Different concentrations of ICG-II (0, 5, 10, 30, 50 μM, respectively) were co-cultured with the cells for 24 hours. Each well was then washed with PBS. And add 10 mL of MTT solution (5 mg ml-1). After 4 h of incubation, remove the medium containing the MTT solution, add DMSO (150 mL) to each well, and shake for 15 min on a shaker to completely dissolve the crystals. Finally, measure the absorbance of each ...

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Abstract

The invention belongs to the field of medicines, and particularly relates to application of a small molecule based on an indole tricarbocyanine structure in preparation of a tumor photothermal therapy drug. A large-conjugated water-soluble indole tricarbocyanine dye compound can be used as a photothermal therapy agent, and has potential development and application prospects. The compound is found to be an excellent tumor photothermal therapy drug, and the application of the compound is not found and reported at present. Through in-vitro property research on the photothermal efficiency of the compound, a basis is provided for clinical application of the small molecule photothermal therapy agent similar to indocyanine green, and a basis is provided for research of the small molecule photothermal therapy agent.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the research on the properties of a small-molecule tumor photothermal treatment drug based on the indole tricarbocyanine structure and its application in treatment. Background technique [0002] Photothermal therapy is a new type of treatment. As we all know, the main clinical treatment methods are surgery, drug chemotherapy and radiotherapy. Surgery can remove early solid tumors, but the resection may not be complete, resulting in residual cancer cells, leading to postoperative cancer metastasis and recurrence. Drug chemotherapy and radiotherapy are the two most commonly used treatment strategies. Although they have inhibitory effects on tumors, they are poorly adaptable to patients due to large toxic side effects, drug tolerance and inherent toxicity of radiation. Based on the shortcomings of the above-mentioned treatment methods, non-invasive photothermal therapy has rece...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61P35/00
CPCA61K41/0052A61P35/00
Inventor 蔡惠明王毅庆陆珍贞朱雄丁晔
Owner NANJING NUOYUAN MEDICAL DEVICES CO LTD
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