Diluent preparation capable of prolonging normal-temperature survival time of boar semen

A technology of diluent and survival period, which is applied in the field of diluent preparation to achieve the effect of stable effect, low cost and high output

Active Publication Date: 2021-08-31
FUJIAN AGRI & FORESTRY UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Single leaves alternate; base of petiole enlarged and clasped; leaves papery, oval to ovate-elliptic, 4-10cm long, 1.5-5cm wide, apex pointed, base broadly cuneate, glabrous, entire, extracted from plumbago It contains plumbagin, β-sitosterol, etc., which have anti-tumor, anti-bacterial, anti-plasmodium, anti-allergic, anti-hyperglycemic effects; , apigenin, coumaric acid and plumbago extract have not been reported

Method used

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  • Diluent preparation capable of prolonging normal-temperature survival time of boar semen
  • Diluent preparation capable of prolonging normal-temperature survival time of boar semen
  • Diluent preparation capable of prolonging normal-temperature survival time of boar semen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 21

[0030] A diluent preparation that can prolong the survival period of porcine semen at room temperature, using an electronic balance to accurately weigh 50.00 g of glucose, 5.00 g of ethylenediaminetetraacetic acid, 0.50 g of tea polyphenols, 15.00 g of sodium citrate dihydrate, and 15.00 g of bicarbonate Dissolve 5.00g of sodium and 3.00g of potassium chloride in 1L of double-distilled water, adjust the pH to 7.5, and the osmotic pressure to 310mOsm / L, and immediately filter with a 0.32μm organic microporous membrane to obtain the basic dilution, and place Store at 25°C for later use;

[0031] Preparation of antioxidants: Take 0.8g of apigenin, 5g of phenethyl caffeate, 2g of coumaric acid and 1g of plumbago extract, heat to 50°C and mix and stir for 10min, cool to 20°C, then heat to 70°C and mix and stir for 8min. get antioxidants;

[0032] Preparation of diluent preparation: Take 0.03 g of antioxidant and add it to 1 L of basic diluent above to prepare a diluent preparation...

Embodiment 2

[0034] A diluent preparation that can prolong the survival period of pig semen at room temperature. Using an electronic balance, accurately weigh 29.50g of glucose, 2.51g of ethylenediaminetetraacetic acid, 0.22g of tea polyphenols, 7.50g of sodium citrate dihydrate, and 7.50g of bicarbonate Dissolve 2.30g of sodium and 1.25g of potassium chloride in 1L double-distilled water, adjust the pH value to 7.2, and the osmotic pressure to 280mOsm / L, and immediately filter it with a 0.25μm organic microporous membrane to obtain the basic dilution, and place Store at 20°C for later use;

[0035] Preparation of antioxidants: Take 0.5g of apigenin, 3g of phenethyl caffeate, 1.7g of coumaric acid and 1g of plumbago extract, heat to 45°C and mix and stir for 6min, cool to 20°C, then heat to 60°C and mix and stir for 5min , to obtain antioxidants;

[0036] Preparation of diluent preparation: Take 0.06 g of antioxidant and add it to 1 L of basic diluent above to prepare a diluent preparatio...

Embodiment 43

[0038] A diluent preparation that can prolong the survival period of porcine semen at room temperature, using an electronic balance to accurately weigh 45.00g of glucose, 4.50g of ethylenediaminetetraacetic acid, 0.40g of tea polyphenols, 12.00g of sodium citrate dihydrate, and 12.00g of bicarbonate Dissolve 3.00g of sodium and 2.50g of potassium chloride in 1L double-distilled water, adjust the pH value to 7.2, and the osmotic pressure to 280mOsm / L, and immediately filter it with a 0.20μm organic microporous membrane to obtain the basic dilution, and place Store at 20°C for later use;

[0039] Preparation of antioxidants: Take 0.5g of apigenin, 3g of phenethyl caffeate, 1.7g of coumaric acid and 1g of plumbago extract, heat to 45°C and mix and stir for 6min, cool to 20°C, then heat to 60°C and mix and stir for 5min , to obtain antioxidants;

[0040] Preparation of diluent preparation: Take 0.09 g of antioxidant and add it to 1 L of basic diluent to prepare a diluent preparat...

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Abstract

The invention provides a diluent preparation capable of prolonging the normal-temperature survival time of boar semen. The diluent preparation is prepared by adding an antioxidant into a basic diluent and mixing the antioxidant with the basic diluent, wherein the mass-volume ratio g / L of the antioxidant to the basic diluent is (0.03-0.15): 1. Each liter of the basic diluent contains the following raw materials by mass: 15.0-50.0 g of glucose, 0.5-5.0 g of ethylenediamine tetraacetic acid, 0.2-0.5 g of tea polyphenol, 5.0-15.0 g of sodium citrate dihydrate, 0.05-5.0 g of sodium bicarbonate and 0.02-3.0 g of potassium chloride. The antioxidant is prepared from apigenin, phenethyl caffeate, cumaric acid and plumbago zeylanica extract in a mass ratio of (0.3-0.8): (2-5): (1.3-2): 1. The basic raw materials and the dosage proportion of the diluent preparation are optimized, the survival time of the boar semen is prolonged under the normal temperature condition, sperm indexes related to the fertilization capacity are maintained, and the diluent preparation has certain guiding significance for obtaining high-quality, high-yield and low-cost boar semen stored at the normal temperature.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a diluent preparation capable of prolonging the normal-temperature survival period of pig semen. Background technique [0002] With the promotion and application of artificial insemination assisted technology in the pig industry, the quality of pig semen is one of the key factors that determine the success or failure of artificial insemination. At present, the storage methods of pig semen mainly include room temperature storage and cryopreservation. Due to the high content of unsaturated fatty acids in the plasma membrane of the porcine sperm head, the ability to resist oxidative damage during cryopreservation is weak, which makes the quality of the thawed sperm (such as Activity rate, plasma membrane functional integrity and acrosome functional integrity) are greatly reduced, which in turn affects production performance. Therefore, the normal temperature storage of pig semen is sti...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226A01N1/021Y02A40/90
Inventor 蓝群谢颖瑜肖天放方绍明林瑞意周劢李家权赵芳露陈德军
Owner FUJIAN AGRI & FORESTRY UNIV
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