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Filtering device and liquid sample treatment system

A filter device and liquid sample technology, applied in the biomedical field, can solve the problems of less than 10% recovery rate, low sensitivity, complicated processing process, etc., and achieve the effect of compressing the filtering processing time, speeding up the filtering speed, and speeding up the detection speed

Pending Publication Date: 2021-08-13
PEKING UNIV THIRD HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most commonly used method is flow cytometry, but the geometric progression of cells that can be detected is generally 10 5 The above is usually only used to detect the number of a specific cell line; it is difficult to detect tumor cells in very rare autologous blood with this method
[0004] In addition, it can also be judged by PCR and cell block pathological methods, but these methods require the processing of autologous blood samples. The processing process includes centrifugation, lysing, washing, and centrifugation again. The number is very limited, the recovery rate is less than 10%, and the number of cells in 1ml is less than 100. When it is lower than 100 / ml, the centrifugation method can hardly find the target cells, and because of the complicated processing process, the identification It takes a long time to destroy the cell structure, it is difficult to retain biological activity, and cannot be used for subsequent identification of tumor cell activity
[0005] Therefore, the current autologous blood transfusion is facing the following problems: First, how to quickly determine whether there are tumor cells in the autologous blood of tumor patients. , the sensitivity is too low, and the detection and filtration takes too long to meet the actual needs; the second is to increase the proportion of tumor cell filtration, such as completely filtering tumor cells or
The lack of methods and devices that can solve the above problems greatly limits the application of autologous blood transfusion technology

Method used

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  • Filtering device and liquid sample treatment system
  • Filtering device and liquid sample treatment system
  • Filtering device and liquid sample treatment system

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Experimental program
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Effect test

Embodiment 1

[0047] The cultured lung cancer A549 cells (National Infrastructure of Cell Line Resource, Beijing, China) were selected, and after cell counting, different numbers of A549 cells were added as target cells into 1ml healthy human whole blood to form 1 cell / ml whole blood, 10 samples / ml whole blood, 100 samples / ml whole blood, and 1000 samples / ml whole blood were compared using centrifugation method and filter structure containing Parylene C membrane, each method was repeated three times, and the average value was taken The experimental results are as follows.

[0048] Tumor cell concentration / ml blood 1 10 100 1000 filter method recovery rate 0 0 15±4.17％ 23±5.22％ centrifugation recovery rate 86±5.29％ 83.2±6.23％ 81.2±3.23％ 79.6±4.67％ Filtration device of the present invention

[0049] The experimental results show that in the experimental group of the filtration structure containing Parylene C membrane, the recovery rate is generally hi...

Embodiment 2

[0051] Use the filtration structure verification of Parylene C membranes with different pore sizes to find the best allowable diameter.

[0052] The filter pores of the Parylene C membrane are customized according to the cell size. If the pore size is too large, tumor cells are easy to leak out and not easy to capture; while the pore size is too small, it will hinder the passage of blood, which is inefficient and easy to block the hole, resulting in research failure. Set a series of pore sizes similar to the size of the target cells, use the samples to pass through filters with different pore sizes one by one, record the recovery rate of target cells and the interception rate of background cells, and select the optimal ratio of high target cell recovery rate and low background cell recovery rate. aperture size. For example, in bacteria-related research, it is found that for bacteria with a diameter of about 1 micron and a length of tens of microns, it is necessary to choose a...

Embodiment 3

[0054] refer to figure 2 , shows a schematic diagram of an example of the filtration device of the present invention, wherein the arrows show the flow direction of the liquid sample; image 3 yes figure 2 An exploded view showing the filter membrane therein, which can be more than one layer, Figure 4 An example of a filter membrane is shown, which also includes a support structure, forming several filter units, each unit comprising a support structure and a filter membrane.

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Abstract

The invention relates to the technical field of biological medicine and advanced manufacturing, and provides a flexible microporous membrane, a filtering device for filtering and removing nucleated cells including tumor cells in autoblood, a detection system comprising the filtering device, a liquid sample treatment system and an autoblood transfusion system. The filtering device comprises a filtering structure containing a Parylene C membrane, the Parylene C membrane contains uniform holes, and the permissible diameter range of the holes is 4 to 8 microns.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a filter device and a liquid sample processing system comprising the filter device. Background technique [0002] During surgical operations, blood transfusion is often required due to the large amount of bleeding. If allogeneic blood is used alone, a considerable part of the operation cannot be carried out as scheduled due to the "blood shortage" that cannot meet the intraoperative needs, and the transfusion of allogeneic blood is likely to inhibit the body's immune response and cause tumors The prognosis of patients is poor, so intraoperative autologous blood transfusion is a method to reduce or even avoid transfusion of allogeneic blood. However, tumor rupture during the operation will increase the number of tumor cells in recovered blood. If the autologous blood washing process cannot completely remove tumor cells, the probability of tumor recurrence and metastasis will...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N15/00G01N21/84B01D63/00
CPCG01N1/34G01N15/00G01N21/84B01D63/00G01N2015/011
Inventor 张小青许川雅刘畅韩彬袁洪福白剑郭向阳王玮李婷宇
Owner PEKING UNIV THIRD HOSPITAL
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