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Large-scale high-yield preparation method of trichoderma harzianum

A technology of Trichoderma harzianum and Trichoderma harzianum, applied in biochemical equipment and methods, methods using spores, methods based on microorganisms, etc., can solve the problems of insufficient spore production and vigorous mycelium growth, and achieve the purpose of increasing spore production, Comprehensive nutrition and good permeability

Active Publication Date: 2021-08-13
山东和众康源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, Trichoderma harzianum tends to have too vigorous mycelium growth and insufficient spore production in the preparation process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A large-scale high-yield preparation method of Trichoderma harzianum comprising the following steps:

[0023] S1. Inoculation: subject the solid fermentation medium to high-temperature sterilization at 125° C., then cool it down, and inoculate Trichoderma harzianum at the same time;

[0024] S2. Fermentation: the inoculated solid fermentation medium is cultured and fermented in a solid fermentation tank to obtain a solid fermentation product;

[0025] S3. Preparation: drying and separating the solid fermentation product in step S2 to obtain Trichoderma harzianum spore powder and fermentation product;

[0026] The technical conditions of the solid fermentation include: the fermentation pH is 5.5-5.8, and the cultivation time is 120 hours.

[0027] Among them, the humidity control process includes: 0h<t≤48h humidity is 80-90%, 48h<t≤108h humidity is 70-80%, 108h<t≤168h humidity is 60-70%.

[0028] Among them, the ventilation control process includes: 0h<t≤24h no ventila...

Embodiment 2

[0035] A large-scale high-yield preparation method of Trichoderma harzianum comprising the following steps:

[0036] S1. Inoculation: subject the solid fermentation medium to high-temperature sterilization at 125° C., then cool it down, and inoculate Trichoderma harzianum at the same time;

[0037] S2. Fermentation: the inoculated solid fermentation medium is cultured and fermented in a solid fermentation tank to obtain a solid fermentation product;

[0038] S3. Preparation: drying and separating the solid fermentation product in step S2 to obtain Trichoderma harzianum spore powder and fermentation product;

[0039] The technical conditions of the solid fermentation include: the fermentation pH is 5.5-5.8, and the cultivation time is 120 hours.

[0040] Wherein, in step S1, the solid fermentation medium comprises the following components by weight percentage: 40% of large leaf bran, 30% of extruded pomace, 8% of sawdust and 75% of adjusted moisture content.

Embodiment 3

[0042] A large-scale high-yield preparation method of Trichoderma harzianum comprising the following steps:

[0043] S1. Inoculation: subject the solid fermentation medium to high-temperature sterilization at 125° C., then cool it down, and inoculate Trichoderma harzianum at the same time;

[0044] S2. Fermentation: the inoculated solid fermentation medium is cultured and fermented in a solid fermentation tank to obtain a solid fermentation product;

[0045] S3. Preparation: drying and separating the solid fermentation product in step S2 to obtain Trichoderma harzianum spore powder and fermentation product;

[0046] The technical conditions of the solid fermentation include: the fermentation pH is 5.5-5.8, and the cultivation time is 120 hours.

[0047] Wherein, in step S1, the solid fermentation medium comprises the following components by weight percentage: 50% of large leaf bran, 40% of extruded pomace, 10% of sawdust and 80% of adjusted moisture content.

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PUM

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Abstract

The invention discloses a large-scale high-yield preparation method of trichoderma harzianum. The preparation method comprises the following steps: S1, performing inoculation, namely carrying out high-temperature sterilization treatment on a solid fermentation culture medium at 125 DEG C, then performing cooling, and inoculating trichoderma harzianum strains at the same time; S2, performing fermentation, namely carrying out culture fermentation on the inoculated solid fermentation culture medium by using a solid fermentation tank to obtain a solid fermentation product; and S3, performing preparation, namely drying and separating the solid fermentation product in the step S2 to obtain trichoderma harzianum spore powder and a fermentation product. According to the preparation method, large-leaf bran, extruded pomace and the wood chips are adopted in the solid fermentation culture medium, the large-leaf bran is high in wheat middling content, comprehensive in nutrition and good in permeability, the wood chips can provide fibers, is mainly used for stimulating cellulase production and has good permeability, and the extruded pomace can provide an acid environment and is good in permeability, therefore, the spore yield and the effective viable count of the trichoderma harzianum can be improved.

Description

technical field [0001] The invention relates to the technical field of Trichoderma harzianum, in particular to a large-scale, high-yield preparation method for Trichoderma harzianum. Background technique [0002] Trichoderma harzianum belongs to anamorphic fungi, Hyphospora, Hyphospora, Hyphoporaceae and Trichoderma according to the traditional classification system. Trichoderma is widely distributed and mainly exists in moist habitats such as forests, slopes and ditches, farmlands and grasslands. Plant residues such as soil, dead branches, fallen leaves and decayed wood, and fruiting bodies of other fungi are its growth substrates. Species are rich and diverse. Trichoderma is an important class of natural biological resources with high economic value. Among the Trichoderma genus, Trichoderma harzianum is the most widely used, and the inoculant made from Trichoderma harzianum has been widely used as a biological control agent, and is widely used in related fields such as a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00C12R1/885
CPCC12N1/14C12N3/00Y02W30/40
Inventor 周长春房华左权张涛娄树军李敦贵
Owner 山东和众康源生物科技有限公司
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