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Chikungunya virus E2 protein rabbit monoclonal antibody and application thereof

A monoclonal antibody and protein technology, applied in the direction of antiviral immunoglobulin, antibody, antiviral agent, etc., can solve the problem of no effective therapeutic drugs and vaccines

Active Publication Date: 2021-08-13
THE NAVAL MEDICAL UNIV OF PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For CHIKV infection, there are currently no effective therapeutic drugs and commercial vaccines

Method used

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  • Chikungunya virus E2 protein rabbit monoclonal antibody and application thereof
  • Chikungunya virus E2 protein rabbit monoclonal antibody and application thereof
  • Chikungunya virus E2 protein rabbit monoclonal antibody and application thereof

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Embodiment Construction

[0039] The implementation of the present invention will be described in detail below in conjunction with the embodiments of the present invention and the accompanying drawings. The following embodiments are implemented on the premise of the technical solution of the present invention, and specific implementation methods and operating procedures are provided, but the protection of the present invention The scope is not limited to the examples described below. All operations related to the CHIKV virus in the present invention are carried out in the three-level biosafety laboratory of the Navy Military Medical University of the Chinese People's Liberation Army.

[0040] 1. Main experimental materials

[0041] 1. CHIKV E2 protein, the ECSA-type CHIKV E2 protein recombinantly expressed by human embryonic kidney 293T cells, is recombinantly expressed by freestyle293F cells grown in suspension culture in serum-free medium by the Biomedical Protection Teaching and Research Office of the...

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Abstract

The invention relates to the technical field of biological medicines, and provides an amino acid sequence of a chikungunya virus (CHIKV) E2 protein rabbit monoclonal antibody and application thereof. The amino acid sequence of the CHIKV E2 protein rabbit monoclonal antibody comprises an amino acid sequence of a heavy chain and an amino acid sequence of a light chain of the antibody. The antibody expressed by mammalian cells can neutralize infection of CHIKV genotypes which are mainly popular in the world on the cells in vitro, and can protect type I interferon receptor genes from knocking out mice to resist fatal CHIKV infection in vivo. The antibody is modified, such as humanized modification or preparation of a single-chain antibody, and is expected to be used for treating severe CHIKV infection.

Description

technical field [0001] The present invention relates to the technical field of biomedical engineering. Specifically, the present invention provides an amino acid sequence of a CHIKV E2 protein rabbit monoclonal antibody and its use in developing therapeutic antibodies against CHIKV infection, including humanized monoclonal antibodies and single-chain antibodies Applications. Background technique [0002] Chikungunya virus (CHIKV) is the pathogen that causes Chikungunya fever (Chikungunyafever). member. CHIKV is an important class of arboviruses. Aedes mosquitoes are its main vector. People are generally susceptible to it. In addition to causing symptoms such as fever and rash, it can often cause chronic arthritis with a course of more than one year. In recent years, it has been reported that CHIKV can cause more serious diseases, including encephalitis and hemorrhagic disorders. At present, CHIKV has spread to more than 100 countries and regions around the world, causing ...

Claims

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Application Information

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IPC IPC(8): C07K16/10C12N15/13A61K39/42A61P31/14
CPCC07K16/1081A61P31/14C07K2317/51C07K2317/515C07K2317/76A61K2039/505Y02A50/30
Inventor 王文唐海琳丁翠玲彭浩然赵平戚中田
Owner THE NAVAL MEDICAL UNIV OF PLA
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