Method for improving quality of mushroom bran feed through multi-strain mixed fermentation

A mixed fermentation, multi-strain technology, applied in microorganism-based methods, animal feed, animal feed, etc., can solve the problems of high content of anti-nutritional factors, high content of crude fiber, difficult to use, etc., to inhibit proliferation and improve absorption. Effectiveness of utilization and maintenance of bacterial flora balance

Pending Publication Date: 2021-08-13
上海源耀农牧科技有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the current problems such as high crude fiber content and low digestibility of fungus bran feed; high water content, easy to mildew; high content of anti-nutritional factors, difficult to use, etc., the present invention provides a safe, stable and edible feed that utilizes mixed fermentation of multiple strains to prepare Method for reducing fiber and anti-nutritional factor content, effectively improving nutritional content and quality fungus chaff feed

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  • Method for improving quality of mushroom bran feed through multi-strain mixed fermentation
  • Method for improving quality of mushroom bran feed through multi-strain mixed fermentation
  • Method for improving quality of mushroom bran feed through multi-strain mixed fermentation

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Embodiment 1

[0026] This embodiment provides a method for improving the quality of bacterium chaff feed by multi-strain mixed fermentation, comprising the following steps:

[0027] Step 1, microbial strain expansion:

[0028] Lactobacillus acidophilus is inoculated into the liquid medium for cultivation, the inoculation amount is 5-15%, and the cultivation temperature is 38-40°C. Among them, the culture medium used for strain culture is MRS medium, and the preparation method is: weigh 20.0g of glucose, 15.0g of peptone, 15.0g of beef extract, 7.5g of yeast extract, 7.5g of sodium acetate, 2.0g of ammonium citrate, hydrogen phosphate Dipotassium 2.0g, magnesium sulfate 0.3g, add water to dissolve to 1000ml, adjust pH=6.8, sterilize at 121°C for 20-30min;

[0029] The Bacillus subtilis is inoculated into the liquid medium for cultivation, the inoculation amount is 5-15%, and the cultivation temperature is 38-40°C. Among them, the culture medium used for strain cultivation is LB medium, and...

Embodiment 2

[0037] The present embodiment adopts single factor experiment to determine the optimal fermentation condition in embodiment 1, and concrete method and result are as follows:

[0038] 1. Method

[0039] The fermentation experiment was carried out under the condition of natural pH with the fungus chaff as the fermentation substrate, and the fermentation temperature was 40°C. After the fermentation is over, it is dried and pulverized at about 75°C and passed through a 40-mesh sieve to measure indicators such as total acid, small peptides, and total viable bacteria before and after fermentation.

[0040] (1) Inoculation amount

[0041] The inoculation amount is 5%, 10%, and 15% as a single factor, and each factor is repeated three times. The ratio of the three strains is 1:1:1, the ratio of material to water is 1:1, the fermentation temperature is 40°C, and the fermentation time is 84h .

[0042] (2) Ratio of material to water

[0043] Set up 3 treatment groups, add distilled ...

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Abstract

The invention provides a method for improving the quality of a mushroom bran feed through multi-strain mixed fermentation, which comprises the following steps: by taking mushroom bran after three times of fruiting as a fermentation substrate and taking lactobacillus acidophilus, saccharomyces cerevisiae and bacillus subtilis which are independently screened and optimized in a laboratory as fermentation strains, performing fermentation under the optimal fermentation condition and the natural pH condition, and after the fermentation is finished, performing low-temperature drying to obtain the fermented mushroom bran feed. The nutritional ingredients and feed quality of the mushroom bran fermented by mixing the three strains of the bacillus subtilis, the lactobacillus acidophilus and the saccharomyces cerevisiae are remarkably improved, the problems that the mushroom bran is poor in palatability, low in absorption and utilization rate, prone to mildewing, not prone to stable storage and the like are greatly solved, and the mushroom bran is a novel feed resource. The fermented mushroom bran feed is rich in probiotic metabolites, and can maintain the balance of gastrointestinal flora of animals, inhibit the proliferation of bad microorganisms in intestinal tracts, improve the absorption and utilization rate of the feed, enhance the immunity of animal organisms, and improve the health level and production performance of the animals.

Description

technical field [0001] The invention relates to the field of feed, in particular to a method for improving the quality of bacterial chaff feed through multi-strain mixed fermentation. Background technique [0002] China is the world's largest producer of edible fungi. According to incomplete statistics, my country's output of edible fungi has reached 39.6 million tons in 2019, accounting for more than 75% of the world's total output of edible fungi. Mushroom chaff is the residue of the culture medium left after the cultivation of various fungi, which contains protein, amino acid and other nutrients. It is estimated that the annual output in my country is about 40 million tons. With the development of industrialization of edible fungi, the output of edible fungi has grown rapidly, and the number of mushroom chaff has also increased year by year. At present, only a small part of these fungus chaffs are developed and utilized as organic fertilizers or cultivation substrates, a...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/37C12N1/20C12N1/18C12R1/865C12R1/125C12R1/23
CPCA23K10/12A23K10/37C12N1/20C12N1/18Y02P60/87
Inventor 丁丽孙中超杨旭高小创刘妍夏志生
Owner 上海源耀农牧科技有限公司
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