Microorganism preservation method for aerogel immobilization

A technology of immobilizing microorganisms and aerogels, which is applied in the field of microbial preservation, can solve the problems of restricting the application of aerogels, poor toughness, and low strength, and achieve the effect of improving storage stability, increasing strength, and good stability

Active Publication Date: 2021-08-10
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, airgel also has certain defects, such as poor toughness, low strength, easy to break, etc., which greatly limits the practical application of airgel.

Method used

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  • Microorganism preservation method for aerogel immobilization
  • Microorganism preservation method for aerogel immobilization
  • Microorganism preservation method for aerogel immobilization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] This embodiment provides a method for preserving microorganisms immobilized in airgel, including:

[0059] 1) Dissolve 10g of sodium alginate (viscosity of 350cp) in water to form an aqueous sodium alginate solution with a mass fraction of 4%, adjust the pH to 3.5 with 0.5mol / L sodium hydroxide solution and 0.5mol / L hydrochloric acid solution , adding 9g mass fraction is 4% potassium permanganate solution, 11g acetic acid / tartaric acid mixed solution (the mass fraction of acetic acid in the solution is 5%, the mass fraction of tartaric acid is 6%), add 2g absolute ethanol, avoid light reaction 14h, add 10g of ethylene glycol to terminate the oxidation reaction, then add 1.5g of sodium chloride to mix well, then add enough ethanol to precipitate, and after suction filtration, the precipitate is dissolved in deionized water, repeat the steps of ethanol precipitation, suction filtration, and dissolution 3 times, Vacuum-dried at 50°C to obtain oxidized sodium alginate; 13 g...

Embodiment 2

[0065] This example provides another airgel-immobilized microbial preservation method. In the process of preparing sodium alginate derivatives, no oxidized sodium alginate is added, and only sodium alginate is used for modification to obtain sodium alginate derivatives. thing; All the other steps are the same as in Example 1.

Embodiment 3

[0067] This example provides another airgel-immobilized microorganism preservation method. In the process of preparing sodium alginate derivatives, no sodium alginate is added, and only oxidized sodium alginate is used for modification to obtain sodium alginate derivatives. thing; All the other steps are the same as in Example 1.

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PUM

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Abstract

The invention provides a microorganism preservation method for aerogel immobilization, and belongs to the technical field of microorganism preservation. The method is particularly as follows: embedding and then preserving microorganisms by using aerogel containing a sodium alginate derivative and polyvinyl alcohol as an immobilization carrier, and using 5-hydroxy-tryptophan for modifying sodium alginate and oxidized sodium alginate in a specific mass ratio, so that the embedding rate of the aerogel on the microorganisms can be obviously improved, the strength of the material can be improved, and the survival rate of the microorganisms is increased. Sodium ascorbate and caffeoyl tartaric acid are added into the aerogel, so that the protection effect on microorganisms can be improved, the survival rate of the microorganisms is further increased, and the preservation stability of the microorganisms can be improved; and in the preservation process, the aerogel and the microorganisms are freeze-dried at the same time in the preparation process, the microorganisms are fixed in the aerogel, the good protection effect on the microorganisms is achieved, the steps are simple, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of microorganism preservation, and in particular relates to an airgel-immobilized microorganism preservation method. Background technique [0002] Microbial strains are important national resources and basic materials for research in microbiology and life sciences. In recent years, more and more microbial strains have been used in food, medicine, agriculture and other fields, and their quality is directly related to the quality and quality of products. Therefore, it is very necessary to choose appropriate preservation methods and technologies. [0003] In order to keep the microbial strains well preserved and play their role to the greatest extent, the preservation of microbial strains is becoming more and more important. At present, the preservation methods of microbial strains mainly include regular transplantation, mineral oil preservation, glycerin preservation, vacuum freeze-drying preservation, dry pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/084C12N11/04C12N1/04
CPCC12N11/10C12N11/084C12N11/04C12N1/04
Inventor 陈庆国刘雨薇刘梅汪涛竺柏康
Owner ZHEJIANG OCEAN UNIV
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