Moisturizing and soothing ganoderma lucidum composite fermentation product as well as preparation method and application thereof
A technology of compound fermented product and Ganoderma lucidum, applied in the field of fermentation
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Embodiment 1
[0048] Example 1 Preparation of Moisturizing and Soothing Ganoderma Lucidum Composite Fermentation Protoplasm
[0049] 1. Preparation of Ganoderma lucidum liquid: Inoculate the Ganoderma lucidum strain with the preservation number CGMCC No.17789 on glucose potato agar medium at 28°C for 7 days to activate, then inoculate the obtained single colony into 100mL glucose potato liquid medium, and inoculate at 28°C 180rpm culture for 7 days to obtain the ganoderma liquid, wherein the volume of mycelium accounts for 80% of the volume of the ganoderma liquid.
[0050] 2. Fermentation substrate preparation: take 0.20g of Ophiopogon japonicus root, 0.10g of Chuanqiong root, 0.25g of holy basil leaves, and 5g of rice, add them to 500g of water, and sterilize at 121°C for 20min to obtain a fermentation substrate; among them, rice accounts for 1 %, Ophiopogon japonicus root accounted for 0.04%, Chuanqiong root accounted for 0.02%, holy basil leaves accounted for 0.05%.
[0051] 3. Obtaini...
Embodiment 2
[0054] Example 2 Moisturizing and Soothing Ganoderma lucidum Composite Fermented Protoplasm as an Application of Cosmetics
[0055] 1. Safety testing of compound fermented puree of moisturizing and soothing Ganoderma lucidum
[0056] The human patch test is mainly used to detect the irritation of cosmetic final products or raw materials. According to the "Hygienic Standards for Cosmetics" (2015), the present disclosure conducts a human body closed patch test on the compound fermented puree obtained in Example 1, aiming to evaluate its potential skin irritation.
[0057] 1. Test object:
[0058] Strictly follow the requirements of the "Diagnostic Criteria and Treatment Principles for Cosmetic Contact Dermatitis" to select subjects. Subjects with scars, port-wine stains, etc. on the skin to be tested, which affect the judgment of the results, and those with highly sensitive constitutions are not allowed to participate in the test. In this experiment, 30 suitable volunteers wer...
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