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Method for cultivating pseudo male gynogenetic megalobrama amblycephala at high temperature

A technique for gynogenesis and bream

Active Publication Date: 2021-08-06
HUNAN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, hormone treatment faces problems such as water pollution and aquatic product safety hazards. It is necessary to further seek safe, efficient and environmentally friendly fish sex reversal technology

Method used

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  • Method for cultivating pseudo male gynogenetic megalobrama amblycephala at high temperature
  • Method for cultivating pseudo male gynogenetic megalobrama amblycephala at high temperature
  • Method for cultivating pseudo male gynogenetic megalobrama amblycephala at high temperature

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Embodiment

[0025] A method for cultivating gynogenetic bream pseudo-male fish at high temperature, comprising the steps of:

[0026] (1) Sperm genetic inactivation: take the male crucian carp (1-2 ages) obtained after artificial induction, extrude the semen and dilute it with Hank's solution at a volume ratio of 1:200, spread it on a petri dish, and irradiate it with a 15W ultraviolet lamp for 2 ~5min to complete the genetic inactivation of crucian carp sperm;

[0027] (2) Artificial gynogenetic development of bream: 2-3-year-old common bream is used as the parent fish to artificially induce labor, and genetically inactivated sperm are used to artificially inseminate the eggs of bream, and the obtained "fertilized eggs" Place it in a petri dish for 3 minutes in static water at 22°C-25°C, then transfer the fertilized eggs to water at 0°C-4°C for cold shock treatment for 22-26 minutes, and then place them in water at 22°C-25°C for static water incubation to obtain artificial females. nucl...

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Abstract

The invention discloses a method for cultivating pseudo male gynogenetic megalobrama amblycephala at high temperature. The method comprises the following steps of cultivating artificial gynogenetic megalobrama amblycephala all-female group fries at 24+ / -1 DEG C for 15-20 days according to a certain specification, transferring the fries into a water body with the temperature of 34+ / -1 DEG C, performing continuous high-temperature induction for 3-4 months, and transferring the fries into a pond for conventional breeding to obtain the pseudo male gynogenetic megalobrama amblycephala. According to the method, exogenous hormones are not used; sex reversal of the gynogenetic megalobrama amblycephala is successfully achieved through a temperature control cultivation method; the survival rate of the gynogenetic megalobrama amblycephala in the high-temperature treatment process is 70%-80%, and the sex reversal rate is 10%-20%; and the method has important significance for expanding propagation of the artificial gynogenetic megalobrama amblycephala and cultivation of new megalobrama amblycephala breeding varieties.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for cultivating gynogenetic giant bream pseudo-male fish by using high temperature. Background technique [0002] Artificial gynogenesis is an effective way for fish genetic improvement, and it plays an important role in the purification and rejuvenation of fish germplasm and the selection of improved species. Bram bream is an important freshwater cultured fish in my country, but the germplasm of bream is degraded at present, which seriously restricts the promotion and development of its culture. The artificial gynogenetic bream produced by gynogenetic technology is an all-female population, and new breeding strains with good quality can be obtained through inbreeding, which has important economic value and social effects in production and application. However, artificial gynogenesis includes double operations of sperm genetic inactivation and chromosome...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 肖亚梅符文彭亮跃刘文彬刘锦辉
Owner HUNAN NORMAL UNIVERSITY
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