All-male macrobrachium rosenbergii seed production method

Abstract

The invention discloses an all-male macrobrachium rosenbergii seed production method. The method comprises the following steps: step 1, design and synthesis of siRNA targeting DMRT1; step 2, transfecting prawn larvae with the obtained siRNA, extracting RNA of the interfered prawn larvae to prepare cDNA through reverse transcription, and detecting expression of DMRT1 and IAG genes after RNA interference by using real-time fluorescent quantitative PCR; step 3, detecting and screening pseudofemale parents; step 4, cultivating and verifying the all-male macrobrachium rosenbergii; and step 5, passage of all-male macrobrachium rosenbergii female parent pseudofemale shrimps. The method can solve the problems of low reversal rate and high mortality rate in the interference process of the all-malemacrobrachium rosenbergii; and the male proportion in offspring for producing shrimps is high, and the culture effect is good.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for seed production of all male Macrobrachium rosenbergii. Background technique [0002] Macrobrachium rosenbergii has a significant difference between male and female. The male shrimp grows faster and has a larger individual, which has a growth advantage. In the market, prawns are popular in many places. Therefore, the breeding of all male Macrobrachium rosenbergii has broad market prospects. Although Israel has begun to use RNAi technology to interfere with the IAG gene to produce "false female" Macrobrachium rosenbergii and the resulting all-male strain. However, due to the high import cost, long intermediate process and high mortality rate, its promotion in the Chinese market is limited; the existing domestic gene seed production method uses microinjection of fertilized eggs or early-stage larval muscle, and the mortality rate is high. The efficiency of false fe...

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Problems solved by technology

However, due to the high import cost, long intermediate process and high mortality rate, its promotion in the Chinese market is limited; the existing domestic gene seed production method uses microinjection of fertilized eggs or early-stage larvae muscle, which has a high mortality rate and is difficult to prepare. The efficiency of false female shrimp is low, which is not conducive to large-scale production; the method of removing the androgenic glands is cumbersome to operate, the reversal efficiency is low, and the mortality rate is high; the market needs a method that can solve the demand for all male Macrobrachium rosenbergii seedlings in production. Method for Seed Production of All Male Macrobrachium rosenbergii

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