Complex microbial inoculant for camel milk fermentation as well as preparation method and application thereof
A compound bacterial agent and fermentation method technology, which is applied in microorganism-based methods, biochemical equipment and methods, bacteria used in food preparation, etc. , not suitable for fermented camel milk and other problems, to achieve the effect of good storage stability, high number of viable bacteria and mild sweetness
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Embodiment 1
[0044] 1. Compound microbial agent for camel milk fermentation
[0045] The above-mentioned composite bacterial agent for camel milk fermentation includes the following strains:
[0046] 4 parts of Lactobacillus plantarum K25, 2 parts of Lactobacillus plantarum KC28, 3 parts of Lactobacillus plantarum D1501, 1.5 parts of Leuconostoc mesentericus SHU1396;
[0047] 2. Preparation method
[0048] The above-mentioned preparation method of the compound bacterial agent for camel milk fermentation, the preparation method comprises the following steps:
[0049] (a) According to 1.5×10 12 Inoculum amount of CFU / L Inoculate each strain into the culture medium and culture at 37°C for 24 hours, centrifuge to obtain the sludge of each strain, wherein,
[0050] The culture medium comprises the following components in weight percentage:
[0051]5% carbon source, 3% nitrogen source, 12% proliferation factor, disodium hydrogen phosphate and potassium dihydrogen phosphate, the balance is UM...
Embodiment 2
[0068] 1. Compound microbial agent for camel milk fermentation
[0069] The above-mentioned composite bacterial agent for camel milk fermentation includes the following strains:
[0070] 6 parts of Lactobacillus plantarum K25, 4 parts of Lactobacillus plantarum KC28, 1 part of Lactobacillus plantarum D1501, 0.5 part of Leuconostoc mesentericus SHU1396;
[0071] 2. Preparation method
[0072] The above-mentioned preparation method of the compound bacterial agent for camel milk fermentation, the preparation method comprises the following steps:
[0073] (a) According to 1.2×10 12 Inoculum amount of CFU / L Inoculate each strain into the culture medium and culture at 37°C for 24 hours, centrifuge to obtain the sludge of each strain, wherein,
[0074] The culture medium comprises the following components in weight percentage:
[0075] Carbon source 3%, nitrogen source 5%, proliferation factor 6%, disodium hydrogen phosphate and potassium dihydrogen phosphate, the balance is UMRS...
Embodiment 3
[0092] 1. Compound microbial agent for camel milk fermentation
[0093] The above-mentioned composite bacterial agent for camel milk fermentation includes the following strains:
[0094] 5 parts of Lactobacillus plantarum K25, 3 parts of Lactobacillus plantarum KC28, 2 parts of Lactobacillus plantarum D1501, 1 part of Leuconostoc mesentericus SHU1396;
[0095] 2. Preparation method
[0096] The above-mentioned preparation method of the compound bacterial agent for camel milk fermentation, the preparation method comprises the following steps:
[0097] (a) According to 1.3×10 12 Inoculum amount of CFU / L Inoculate each strain into the culture medium and culture at 37°C for 24 hours, centrifuge to obtain the sludge of each strain, wherein,
[0098] The culture medium comprises the following components in weight percentage:
[0099] 4% carbon source, 4% nitrogen source, 10% proliferation factor, disodium hydrogen phosphate and potassium dihydrogen phosphate, the balance is UMRS...
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