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Method for rapidly detecting total potassium content of urban green land soil by using archaea molecular marker OTU240

A potassium content and soil technology, which is applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of long measurement period and cumbersome measurement steps, and achieve short time required and low labor cost. Low, to achieve the effect of rapid automatic detection

Active Publication Date: 2021-07-23
SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the determination of total potassium content in soil mainly uses sodium hydroxide alkali fusion or perchloric acid-hydrofluoric acid digestion, flame photometry and flame atomic absorption method. The determination steps are cumbersome and the determination period is long.

Method used

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  • Method for rapidly detecting total potassium content of urban green land soil by using archaea molecular marker OTU240
  • Method for rapidly detecting total potassium content of urban green land soil by using archaea molecular marker OTU240
  • Method for rapidly detecting total potassium content of urban green land soil by using archaea molecular marker OTU240

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1, find the OUT relevant with soil total potassium content

[0060] 1. Collection of soil samples

[0061] In November 2017, research plots were set up in representative parks and green spaces in 16 administrative districts of Shanghai.

[0062] Sampling method: Soil sample collection follows the sampling principle of multi-point mixing. Select 8 sampling points for each plot, and use a 2.5cm diameter soil drill to collect 0-20cm surface soil respectively, and then mix them into 1 soil sample.

[0063] A total of 76 soil samples were collected.

[0064] The soil samples were mixed evenly and passed through a 2mm sieve to remove plant roots, gravel and other debris. Each soil sample was then divided into two. One sample was air-dried naturally, and then sampled for the determination of soil chemical properties in step 2; the other sample was stored at -80°C, and then sampled for the extraction of total soil DNA in step 3.

[0065] 2. Analysis and determina...

Embodiment 2

[0087] Embodiment 2, establishing the linear relationship between OTU and soil chemical characteristics

[0088] 1. Archaeal Marker Gene Probe Design

[0089] According to the sequencing results, a probe for detecting OTU240 was designed.

[0090] Probe240-1 (SEQ ID NO: 1 of the Sequence Listing): 5'-TGCCGGCTTGGTGTCGCGGA-3'.

[0091] Probe240-2: 5'-AGTGAAGATGTTAAGCCGG-3'.

[0092] The Probe240-1 probe is a Taqman probe with a fluorescent group FAM at the 5' end and a fluorescent quencher TAMRA at the 3' end. The Probe240-2 probe is a Taqman probe with a fluorescent group FAM at the 5' end and a fluorescent quencher TAMRA at the 3' end.

[0093] According to the sequencing results, a probe for detecting OTU72 was designed.

[0094] Probe72-1: 5'-CAGGTGCGGTGCGACCGCA-3'.

[0095] Probe72-2: 5'-AACTTGAAGCGAGTCACCTG-3'.

[0096] The Probe72-1 probe is a Taqman probe with a fluorescent group FAM at the 5' end and a fluorescent quencher TAMRA at the 3' end. The Probe72-2 probe...

Embodiment 3

[0118] Example 3, Molecular detection of total potassium content in unknown urban green space soil samples

[0119] Three green spaces were randomly selected in Shanghai Botanical Garden, Nanjing Xuanwu Lake Park and Suzhou Wujiang Park respectively.

[0120] Sampling method: Soil sample collection follows the sampling principle of multi-point mixing. Select 8 sampling points for each plot, and use a 2.5cm diameter soil drill to collect 0-20cm surface soil respectively, and then mix them into 1 soil sample.

[0121] The soil samples were mixed evenly and passed through a 2mm sieve to remove plant roots, gravel and other debris. Each soil sample was then divided into two. One sample was air-dried naturally, and then sampled to detect the total potassium content to obtain the measured value of the total potassium content (in g / kg); the other sample was stored at -80°C, and then sampled for total soil DNA extraction.

[0122] 1. Extract the total DNA from the soil sample. Usin...

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Abstract

The invention discloses a method for rapidly detecting the total potassium content of urban green land soil by using an archaea molecular marker OTU240. The invention provides a DNA molecule (probe) as shown in sequence 1 in a sequence table. The invention also protects the application of the probe in detection or auxiliary detection of the total potassium content of soil. The invention also protects the application of the probe in comparison of the total potassium content of soil in different land parcels. The method provided by the invention is used for detecting the total potassium content of the soil or comparing the total potassium content of the soil in different land parcels, and has the advantages of small sample demand, no need of pretreatment, short required time, low labor cost and capability of realizing rapid and automatic detection of a large batch of samples. The method has important application and popularization values for evaluation of soil samples.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for rapidly detecting the total potassium content of urban greenbelt soil by using the archaeal molecular marker OTU240. Background technique [0002] Urban landscaping construction is an important part of the construction of ecological civilization and the construction of a beautiful China. It plays an important role in optimizing the urban environment, improving the quality of life of the people, and promoting the sustainable development of the city. As the material basis of urban ecological environment, the quality of urban green space soil directly affects the healthy growth of plants, ecological benefits, and landscape functions, and determines the quality of urban green space to a certain extent. Therefore, scientific and objective monitoring and evaluation of the soil quality of urban green space is an important reference and basis for urban green space management. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6851C12N15/11
CPCC12Q1/6851C12Q2531/113C12Q2561/101C12Q2537/165
Inventor 张浪韩继刚张维维张琪王香春
Owner SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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