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Method for preparing electron mediator

An electronic mediator, solid technology, applied in the direction of electrochemical variables of materials, etc., can solve the problems of low iron content, inaccurate measurement of the concentration of the substance to be measured, and low sensor sensitivity.

Pending Publication Date: 2021-07-09
可孚医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amount of grafted ferrocene is too low, resulting in low sensor sensitivity, high noise, inaccurate measurement of the concentration of the analyte, and shortened response time

Method used

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  • Method for preparing electron mediator
  • Method for preparing electron mediator
  • Method for preparing electron mediator

Examples

Experimental program
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preparation example Construction

[0018] A method for preparing an electron mediator, comprising the following manufacturing steps:

[0019] Step 1: Fully dissolve polyethyleneimine PEI (preferably with an average molecular weight of 10000Da-1000000Da) in a solvent, the solvent can be one of methanol, ethanol, tetrahydrofuran, etc., to obtain a PEI solution;

[0020] Step 2: Dissolve ferrocene formaldehyde, ferrocene diformaldehyde or its homologues in a solvent, and stir to fully dissolve it. The solvent can be one of organic solvents such as methanol, ethanol, methylene chloride, tetrahydrofuran, etc. , preferably the same solvent as in step (1);

[0021] Step 3: Add ferrocene formaldehyde, ferrocene dicarbaldehyde or its homologue solution into PEI solution, and react at 0°C-100°C for 1-24 hours, wherein the reaction temperature is preferably room temperature;

[0022] Step 4: cooling the reaction solution to 0°C-20°C, preferably to 0°C-5°C;

[0023] Step 5: adding sodium borohydride into the reaction sol...

specific Embodiment 1

[0030] 1200 mg of polyetherimide (PEI) was dissolved in 40 ml of methanol, and 72 mg of ferrocene formaldehyde was dissolved in 12 ml of methanol. Slowly add the ferrocene formaldehyde solution into the PEI methanol solution, and react at room temperature for 3 hours, then place the reaction solution in the refrigerator for 15 minutes to cool it down to about 4°C. Add 2000 mg of sodium borohydride to the cooled reaction solution, and stir to continue the reaction for 1 h. After the reaction was completed, the reaction solution was concentrated to dryness. Then add diethyl ether and soak for 24 hours, and dry after soaking to obtain 1250 mg of electron mediator.

[0031] Among them, the infrared spectrum of the obtained electron mediator is as follows figure 1 shown.

[0032] Further analyze the content of grafted ferrocene in this compound, such as figure 2 As shown, the content is more than 50%.

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Abstract

The invention discloses a novel method for preparing an electronic mediator capable of being used for an electrochemical biosensor. The electronic mediator is prepared by carrying out polymerization reaction on polyethylene imine (PEI) and one of ferrocene carboxaldehyde, ferrocene dicarboxaldehyde or homologues of ferrocene dicarboxaldehyde. Compared with the prior art, the sensitivity of the sensor can be improved, the detection noise of the sensor can be reduced, and longer response time can be obtained.

Description

technical field [0001] The invention relates to an electron mediator, in particular to a preparation method of a ferrocene electron mediator. Background technique [0002] With the advancement of technology, electrochemical biosensors, as an important sensor, appear in people's production and life in large numbers, such as modern glucose biosensors for diabetes detection. An electrochemical biosensor is an instrument that is sensitive to biological substances and converts their concentration into electrical signals for detection. The substance to be measured enters the bioactive material through diffusion, and undergoes a biochemical reaction with the electronic mediator through molecular recognition. The information generated is then converted into a quantifiable and processable signal by the corresponding physical or chemical transducer, and then passed through The secondary meter is amplified and output to know the concentration of the analyte. In the electrochemical bi...

Claims

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Application Information

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IPC IPC(8): C08G73/04G01N27/26
CPCC08G73/0206G01N27/26
Inventor 张敏
Owner 可孚医疗科技股份有限公司
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