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A method for constructing a diabetic zebrafish model

A technology for zebrafish and diabetes, which is applied in the field of biological modeling, can solve the problems of building a diabetic zebrafish model, long culture cycle, and long modeling time, etc., and achieves excellent application prospects, short modeling time, and consumables low cost effect

Active Publication Date: 2022-08-02
WENZHOU UNIV
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Problems solved by technology

[0003] However, the main disadvantages of the current zebrafish model are high mortality and long modeling time. These defects are also the main problems that have not been overcome in modeling.
Patent CN201810767264.8 discloses a breeding method and device for improving the survival rate of zebrafish juveniles. The breeding method can significantly improve the survival rate of zebrafish juveniles with a specific device, but the culture period is longer. And did not give the inspiration to construct the diabetic zebrafish model
[0004] Therefore, there is an urgent need for a diabetic zebrafish model to be used in the research of diabetes-related drugs, and to overcome the above-mentioned technical problems such as high mortality and long modeling time.

Method used

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  • A method for constructing a diabetic zebrafish model
  • A method for constructing a diabetic zebrafish model
  • A method for constructing a diabetic zebrafish model

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specific Embodiment

[0030] 1. Experimental animals

[0031] Zebrafish: wild type, Institute of Hydrobiology, Chinese Academy of Sciences (select healthy adult zebrafish over 5 months old; 14 hours of light per day and 10 hours of darkness); the requirements for healthy zebrafish here: the blood sugar index is normal (that is, blood sugar The concentration is between 2.8mmol / L-6.1mmol / L).

[0032] Artemia hatching eggs (-20 ℃ storage): Tianjin Fengnian Aquaculture Co., Ltd.

[0033] 2. Experimental reagents

[0034] Glucose (analytical grade): Tianjin Beilian Fine Chemicals Development Co., Ltd.;

[0035] Artemia hatching salt: Tianjin Fengnian Aquaculture Co., Ltd.;

[0036] Cool tap water after boiling.

[0037] 3. Experimental equipment

[0038] Artemia incubator: volume is 1700ml, (Jian Keshan Technology Co., Ltd.);

[0039] Zebrafish culture circulating water system: water temperature 28℃, pH7-8, conductivity: 500-800μS / cm, salinity: 0.25-0.5% (Shanghai Feixi Biotechnology Co., Ltd.); ...

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Abstract

The invention relates to the field of biological modeling, in particular to a method for constructing a diabetic zebrafish model. The invention induces the construction of a diabetic zebrafish model by increasing the content of sugar in the zebrafish feed, thereby realizing the determinability of the dosage. The modeling of the invention has the advantages of low mortality, short modeling time, controllable dosage of administration, simple and convenient method, and low cost of consumables required in the modeling process, and has excellent application prospects.

Description

technical field [0001] The invention relates to the field of biological modeling, in particular to a method for constructing a diabetic zebrafish model. Background technique [0002] Diabetes includes various types. Clinically, the glucose content in fasting blood exceeds 7.1 mmol / L as an indicator. The zebrafish diabetes model is often used in the prior art to study the hypoglycemic efficacy of hypoglycemic drugs. Lao Qiaocong et al. Zebrafish Diabetes Model to Evaluate the Hypoglycemic Efficacy of Commonly Used Hypoglycemic Drugs”, select wild-type AB lineage zebrafish juveniles, and use alloxan combined with glucose as an inducer to establish a zebrafish diabetes model; use this model to evaluate metformin, acarbo Hypoglycemic efficacy of sugar, sitagliptin and glibenuria. [0003] However, the main shortcomings of the current zebrafish model are high mortality and long modeling time, which are also the main problems that have not been overcome by modeling. Patent CN201...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10A01K61/59A01K61/17
CPCA01K61/10A01K61/59A01K61/17Y02A40/81
Inventor 王磊孙达金立波彭仁义李瑶琦
Owner WENZHOU UNIV
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