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Method for analyzing metabolic behaviors of PCB95 and metabolites thereof in chicken liver microsomes

A technology of PCB95 and 4-OH-PCB95, which is applied in the field of environmental toxicology, can solve problems such as retention and no relevant reports on chicken liver microsome research

Active Publication Date: 2021-07-02
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research is mainly on the distribution of PCB95 and the identification of metabolites in mice, dogs, rabbits and other microsomes. At the same time, there is little research on the metabolic dynamics of PCB95 and its metabolites in chickens. Studies don't even report on it

Method used

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  • Method for analyzing metabolic behaviors of PCB95 and metabolites thereof in chicken liver microsomes
  • Method for analyzing metabolic behaviors of PCB95 and metabolites thereof in chicken liver microsomes
  • Method for analyzing metabolic behaviors of PCB95 and metabolites thereof in chicken liver microsomes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Chicken Liver Microsome Incubation System Establishment

[0044] 1.1 Incubation solution preparation:

[0045]Prepare 50mmol Tris-HCl buffer solution with pure water; dissolve NADPH-Na4 with 50mmol Tris-HCl buffer solution so that the final concentration in the entire incubation system is 1mmol; MgCl 2 Dissolve with 50mmol Tris-HCl buffer solution, so that the final concentration in the entire incubation system is 5mmol. The above solutions are ready-to-use and stored at 4°C before use.

[0046] 1.2 Standard product preparation:

[0047] PCB95, 4-OH-PCB95 and 4-MeO-PCB95 were purchased from AccuStandard, all of which were white solid powders, dissolved in dimethyl sulfoxide (DMSO for short), stored in a cool cabinet, and used now Ready to match.

[0048] 1.3 Incubation system preparation:

[0049] Chicken liver microsomes were purchased from Wuhan Platt Biomedical Technology Co., Ltd., with a protein concentration of 20 mg / mL, stored at -80°C, and the num...

Embodiment 2

[0085] Example 2 Toxicity difference and metabolic behavior analysis of PCB95 and its metabolites

[0086] 1. Toxicity difference analysis

[0087] 1.1 Incubation concentration:

[0088] The chicken liver microsomal protein concentration was 0.1 mg / mL, the concentrations of PCB95 and MeO-PCB95 were 1, 5, 10, 20 and 50 μg / mL, and the concentrations of OH-PCB95 were 1, 5 and 10 μg / mL.

[0089] 1.2 Incubation scheme:

[0090] The incubation system was set for each compound according to its incubation concentration, and each system was prepared according to 5 time points of 0, 1, 2, 4 and 6h, and each time point was 3 parallels, and each parallel was prepared in a total volume of 200 μl.

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PUM

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Abstract

The invention provides a method for analyzing metabolic behaviors of PCB95 and metabolites thereof in chicken liver microsomes, and relates to the technical field of environmental toxicology. The method comprises the steps of (a) establishing a chicken liver microsome incubation system; (b) performing incubation metabolism on PCB95 with different concentrations and metabolites thereof in the chicken liver microsome incubation liquid; (c) determining the contents of PCB95 and metabolites thereof in the incubation liquid by using a gas chromatograph and an electron capture detector; and (d) analyzing and evaluating characteristic metabolic behaviors and toxicity differences of PCB95 and metabolites thereof in chicken liver microsomes according to quantitative results. The method can be used for simultaneously analyzing the metabolism conditions of PCB95 and metabolites thereof in chicken liver microsomes, and is simple to operate and high in analysis speed.

Description

technical field [0001] The invention relates to the technical field of environmental toxicology, in particular to a method for analyzing the metabolic behavior of PCB95 and its metabolites in chicken liver microsomes. Background technique [0002] Polychlorinated biphenyls (PCBs) are persistent organic pollutants formed by multiple chlorination of biphenyls, which have been detected in organisms and natural environments, and can have a series of adverse effects on organisms. Including causing cancer and causing adverse effects on nervous, reproductive, endocrine and other systems. Therefore, PCBs have been banned from industrial production in the late 1970s, but they still exist in the environment as industrial by-products. Not only that, due to the bioaccumulation of PCBs, they can be metabolized into hydroxy metabolites, methoxy metabolites and methylsulfonyl metabolites in organisms, among which hydroxy metabolites are the main products. [0003] my country is the large...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/70
CPCG01N30/02G01N30/06G01N30/70
Inventor 邱静廖光琴张崴
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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