Method for detecting biological available mercury in soil

A detection method, soil biology technology, applied in the direction of measurement device, test sample preparation, material excitation analysis, etc., can solve the problems of detection difficulties, and achieve the effect of reliable method, high precision and good stability

Pending Publication Date: 2021-07-02
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above-mentioned technical problems, the present invention provides a method for detecting soil bioavailable mercury. The present invention ensures the effective and efficient extraction of soil bioavailable mercury through a specially prepared soil available mercury extractant that is significantly related to crop absorption. Accurate and stable, can effectively solve the difficult problem of soil bioavailable mercury detection, and help solve the problem of judging the safety of soil planted crops

Method used

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  • Method for detecting biological available mercury in soil
  • Method for detecting biological available mercury in soil
  • Method for detecting biological available mercury in soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: Comparison of extraction effects of different extractants

[0052] Choose existing common extractant hydrochloric acid, calcium chloride, ammonium acetate, diethyltriaminepentaacetic acid, and sulfhydryl acetic acid+disodium hydrogen phosphate and extractant sulfhydryl acetic acid+calcium chloride of the present invention carry out extraction effect test.

[0053] 1. Pretreatment of soil samples:

[0054] Select 8 samples with low, medium and high concentrations, accurately weigh 5.00g of soil samples passed through a 2mm aperture nylon sieve, put them in a dry 250mL plastic bottle, add 0.1mol / L hydrochloric acid, 0.1mol / L calcium chloride, 1mol / L L ammonium acetate, 0.005mol / L diethyltriaminepentaacetic acid, 0.03% sulfhydrylacetic acid + 1 / 15mol L -1 Disodium hydrogen phosphate, 0.03% sulfhydryl acetic acid + 0.1mol / L CaCl 2 Extract 50mL, cap the bottle tightly, shake at 25°C±2°C at a shaking speed of 180±20rpm / min for 2h, centrifuge and filter immedi...

Embodiment 2

[0074] Embodiment 2: extract adds hydrochloric acid preservation test

[0075] 1. Pretreatment of soil samples:

[0076] Choose low and high two concentration samples (No. 1 soil and No. 2 soil), accurately take by weighing 5.00g of the soil sample of 2mm aperture nylon sieve, place in the plastic bottle of dry 250mL, add and contain 0.03wt% sulfhydryl acetic acid, 0.1mol / L CaCl 2 Water extractant 50mL, cap the bottle tightly, shake at a temperature of 25°C±2°C at a speed of 180±20rpm / min for 2h, centrifuge and filter immediately, take the supernatant and add hydrochloric acid (preferably Grade pure) and no hydrochloric acid detection.

[0077] 2. Preparation of standard working solution:

[0078] Dilute the mercury standard solution 1000mg / L with the certificate of standard substance with 5% hydrochloric acid step by step to 10.0μg / L standard working solution. Pipette 0.00, 2.5, 5.00, 10.0, 15.0, 20.0mL of mercury standard working solution into a 100mL volumetric flask, d...

Embodiment 3

[0095] Embodiment 3: detection method precision experiment

[0096] 1. Pretreatment of soil samples:

[0097] Select three samples with low, medium and high concentrations, accurately weigh 5.00g of the soil sample passed through a nylon sieve with an aperture of 2mm, place it in a dry 250mL plastic bottle, add 0.03wt% sulfhydryl acetic acid, 0.1mol / L CaCl 2Water extractant 50mL, cap the bottle tightly, shake at 25°C±2°C at a shaking speed of 180±20rpm / min for 2h, centrifuge and filter immediately, take the supernatant and add hydrochloric acid (premium grade) in a proportion of 5wt% Pure), for testing.

[0098] 2. Preparation of standard working solution:

[0099] Dilute the mercury standard solution 1000mg / L with the certificate of standard substance with 5% hydrochloric acid step by step to 10.0μg / L standard working solution. Pipette 0.00, 2.5, 5.00, 10.0, 15.0, 20.0mL of mercury standard working solution into a 100mL volumetric flask, dilute to the marked line with extr...

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Abstract

The invention relates to the technical field of soil detection, and discloses a method for detecting biological available mercury in soil, which comprises the following steps of 1) collecting a soil sample, 2) air-drying the soil sample, 3) pre-treating the soil sample by using an extracting agent, 4) drawing a standard curve, 5) sample detection: detecting a to-be-detected sample to obtain fluorescence intensity of the to-be-detected sample, and substituting the fluorescence intensity into the standard curve to obtain a mercury concentration value, if the mercury concentration value exceeds the standard curve range, diluting the mercury concentration value with an extracting agent and then detecting, and substituting the obtained mercury concentration value into a formula to calculate the content of the biological available mercury in the soil sample. The method has the advantages of high sensitivity, good accuracy and stability and small error, and can realize the content detection of the biological available mercury with good correlation with crops in the soil.

Description

technical field [0001] The invention relates to the technical field of soil detection, in particular to a method for detecting soil biologically available mercury. Background technique [0002] Mercury is a highly toxic heavy metal element and one of the pollutants of global concern. The soil in many places at home and abroad has been polluted by mercury to varying degrees. Mercury pollution in soil is latent and can pose a threat to human health through the food chain, such as amyotrophic lateral sclerosis, Alzheimer's disease and Parkinson's disease. Cardiovascular system poses a threat. Mercury in soil exists in many forms, and the available mercury content that plants can absorb (including water-soluble, substitutable and chelated mercury) is crucial to the safety of agricultural products. Studies have confirmed that it is difficult to evaluate the soil pollution status and its impact on the safety of agricultural products with the index of total heavy metals in soil,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/28G01N1/38
CPCG01N21/6404G01N1/28G01N1/38
Inventor 叶雪珠赵首萍肖文丹张棋陈德胡静
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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