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A lyophilized protective agent for 2019-ncov multiplex amplification reaction reagent and its application

A technology of 2019-ncov and freeze-drying protective agent, which is applied in the direction of microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems that cannot meet the requirements of long-term storage of 2019-nCov amplification reaction reagents, short storage time, etc. , to achieve good freeze-drying protection effect, high sensitivity, and improve the effect of storage time

Active Publication Date: 2021-11-26
INTEGRATED BIOSYSTEMS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the common problem in the current freeze-drying technology is that its storage time is short, which cannot meet the requirements for long-term storage of 2019-nCov amplification reaction reagents

Method used

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  • A lyophilized protective agent for 2019-ncov multiplex amplification reaction reagent and its application
  • A lyophilized protective agent for 2019-ncov multiplex amplification reaction reagent and its application
  • A lyophilized protective agent for 2019-ncov multiplex amplification reaction reagent and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A method for preparing 2019-nCov multiple amplification reaction reagent freeze-dried sheets, the reagent composition is shown in Table 3.

[0054] Table 3 embodiment 1 reagent composition

[0055]

[0056]

[0057]

[0058] The preparation method of 2019-nCov multiple amplification reaction reagent lyophilized sheet comprises the following steps:

[0059] (1) Mix trehalose, mannitol, pullulan, bovine serum albumin, surfactant, defoamer, sodium salicylate, chondroitin sulfate, versicolor polysaccharide, and hyaluronic acid in the formula, and mix evenly. Made into lyoprotectant;

[0060] (2) Mix primers, probes, and deoxynucleotide triphosphates evenly to make amplification reaction reagents;

[0061] (3) After mixing the lyoprotectant and the amplification reaction reagent evenly, adding a buffer and an enzyme mixture to make a lyophilized reagent;

[0062] (4) Adding the freeze-dried reagent to the silica gel mold, transferring the mold to a freeze dryer f...

Embodiment 2

[0067] A method for preparing 2019-nCov multiple amplification reaction reagent freeze-dried sheets, the reagent composition is shown in Table 5.

[0068] Table 5 embodiment 2 reagent composition

[0069]

[0070]

[0071] The preparation method of 2019-nCov multiple amplification reaction reagent lyophilized sheet comprises the following steps:

[0072] (1) Mix trehalose, mannitol, pullulan, bovine serum albumin, surfactant, defoamer, sodium salicylate, chondroitin sulfate, versicolor polysaccharide, and hyaluronic acid in the formula, and mix evenly. Made into lyoprotectant;

[0073] (2) Mix primers, probes, and deoxynucleotide triphosphates evenly to make amplification reaction reagents;

[0074] (3) After mixing the lyoprotectant and the amplification reaction reagent evenly, adding a buffer and an enzyme mixture to make a lyophilized reagent;

[0075] (4) Add the lyophilized reagent into the silica gel mold, transfer the mold to a lyophilizer for lyophilization, ...

Embodiment 3

[0085] A method for preparing 2019-nCov multiple amplification reaction reagent freeze-dried sheets, the reagent composition is shown in Table 7.

[0086] Table 7 embodiment 3 reagent composition

[0087]

[0088]

[0089]

[0090] The preparation method of 2019-nCov multiple amplification reaction reagent lyophilized sheet comprises the following steps:

[0091] (1) Mix trehalose, mannitol, pullulan, bovine serum albumin, surfactant, defoamer, sodium salicylate, chondroitin sulfate, versicolor polysaccharide, and hyaluronic acid in the formula, and mix evenly. Made into lyoprotectant;

[0092] (2) Mix primers, probes, and deoxynucleotide triphosphates evenly to make amplification reaction reagents;

[0093] (3) After mixing the lyoprotectant and the amplification reaction reagent evenly, adding a buffer and an enzyme mixture to make a lyophilized reagent;

[0094] (4) Add the lyophilized reagent into the silica gel mold, transfer the mold to a lyophilizer for lyop...

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Abstract

The invention belongs to the technical field of medicine preparation, and specifically relates to a lyoprotectant for 2019‑nCov multiple amplification reaction reagents and an application thereof. The lyoprotectant for 2019-nCov multiple amplification reaction reagents includes the following components: trehalose 1-20%, mannitol 4-20%, pullulan 0.1-4%, bovine serum albumin 0.0001‑1%, surfactant 0.01‑0.5%, defoamer 0.01‑0.1%, sodium salicylate 0.1‑1%, chondroitin sulfate 0.5‑5%, versicolor polysaccharide 0.1‑2%, hyaluronic acid 1 -5%. The freeze-drying protective agent has better freeze-drying effect and can effectively improve the storage time of amplification reaction reagents. Its freeze-drying method is simple and easy.

Description

technical field [0001] The invention belongs to the technical field of medicine preparation, and in particular relates to a lyoprotectant for 2019-nCov multiple amplification reaction reagents and its application. Background technique [0002] Freeze drying refers to a drying method that freezes the dried water-containing material into a solid, and uses the sublimation performance of water under low temperature and reduced pressure to dehydrate the material at low temperature to achieve the purpose of drying. [0003] Freeze-drying technology has many advantages, such as: many heat-sensitive substances will not be denatured or inactivated; during the freeze-drying process, the growth of microorganisms and the action of enzymes cannot be carried out, so the original properties can be maintained; the volume is almost unchanged, The original structure is maintained, no condensation occurs; drying is carried out under vacuum, with very little oxygen, and some easily oxidized sub...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/686
CPCC12Q1/686C12Q1/701C12Q2600/16C12Q2537/143C12Q2527/125C12Q2521/107C12Q2565/629
Inventor 贾欣月高静张瑜蔡亦梅金鑫浩任鲁风
Owner INTEGRATED BIOSYSTEMS CO LTD
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