Production method of lentinus edodes liquid stock seed and lentinus edodes liquid stock seed

Abstract

The invention provides a production method of lentinus edodes liquid stock seeds and lentinus edodes liquid stock seeds, and relates to the technical field of lentinus edodes liquid fermentation. According to the production method of the lentinus edodes liquid stock seeds, a liquid culture medium and the lentinus edodes stock seeds are stirred and cultured in a magnetic stirring mode, an existing shaking table operation mode is replaced, hyphae can be continuously broken through shearing force generated by rotation of a magnetic rotor, growing points are increased, and the culture time is remarkably shortened; the liquid stock seeds produced by magnetic stirring have small and more hyphae and strong vitality, and when the next-level liquid strain is transferred, the germination points are more, so that the fermentation time of the next-level strain can be shortened. In addition, compared with an operation mode of a shaking table, the magnetic stirrer is convenient to use and low in input cost.

Description

technical field [0001] The invention relates to the technical field of liquid fermentation of shiitake mushrooms, in particular to a production method of liquid original seeds of shiitake mushrooms and liquid original seeds of shiitake mushrooms. Background technique [0002] The traditional production process of shiitake mushroom strains mainly uses solid strains, which has the disadvantages of large amount of seeds, high labor intensity, cumbersome process, high contamination rate of bacteria, long growth cycle, inconsistent bacterial age, and large area for strain cultivation. , which to some extent restricts the transformation and upgrading of the shiitake mushroom industry. The liquid strains have the advantages of short seed production time, high inoculation efficiency, fast colonization cover, short germination cycle, uniform bacterial age, low pollution rate and low production cost (see Table 1 for details), and are especially suitable for large-scale edible fungi. ...

AI Technical Summary

Problems solved by technology

Because the mother seed of shiitake mushroom is harder than other edible fungi, the shaker cannot quickly and evenly disperse the seed block into the liquid medium

Therefore, in the shaker culture process, the mother seed block is deposited in the bottom of the liquid medium in the form of large particles, which has problems such as long fermentation time, poor strain activity, and excessively large bacterial ball particles, which also makes the bacterial balls in the bottom of the liquid medium. Partial deposition, germination points decrease when transferring to the next level of bacteria

In addition, the temperature-controlled shaker is expensive, which increases the input cost of the enterprise

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