Composite microbial preparation and its application in sewage treatment
A technology of composite microorganisms and sewage treatment methods, applied in the field of microbial preparations, to achieve the effect of deodorizing sewage transparency, increasing sewage transparency, high metabolic substrate binding ability and catalytic activity
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Embodiment 1
[0083] 1. Fiber iron (III) complex (F-Fe), prepared by the following method:
[0084] Add fiber and trisodium citrate to 2mol / L FeCl 3 In the aqueous solution, the coordination reaction was carried out at 65° C. and 200 rpm stirring speed for 1.5 h, washed with distilled water for 4 times, and dried to obtain the fibrous iron (III) complex. of which fiber, trisodium citrate and FeCl 3 The dosage ratio of the aqueous solution is 1 g: 0.5 g: 20 mL.
[0085] 2. Citric acid modified fiber iron (III) complex (CAF-Fe), prepared by the following method:
[0086] 1) Add the fiber and sodium dihydrogen phosphate into a 2mol / L citric acid aqueous solution, carry out a coordination reaction at 70°C and a stirring speed of 200rpm for 2h, wash with distilled water 4 times, and dry to obtain an esterified modified fiber; fiber; , the dosage ratio of sodium dihydrogen phosphate and citric acid aqueous solution is 1g:0.12:20mL;
[0087] 2) Add the esterified fiber and trisodium citrate to...
Embodiment 2
[0117] 1. Bacteria
[0118] Paracoccus denitrificans, ATCC 13543; Bacillus amyloliquefaciens, ATCC 23843; Bacillus coagulans, ATCC 15949; Bacillus halotolerant, ATCC 05376; Pseudomonas stutzeri, ATCC 83626; Alkali bacteria, ATCC 52498; Achromobacter xyloseoxidizing, ATCC 15327; Vibrio fibrous, ATCC 13127;
[0119] Those skilled in the art can select appropriate medium and expansion culture method according to common sense, so that the above-mentioned number of viable bacteria can reach 3-5×10 8 pcs / mL.
[0120] 2. A composite microbial preparation comprising:
[0121] Microorganisms, in parts by weight, including 5.0 parts of Bacillus amyloliquefaciens, 2.5 parts of Bacillus coagulans, 1.0 parts of Halobacterium, 3.2 parts of Pseudomonas nitrogenogeni, 4.0 parts of Pseudomonas stutzeri, 2.5 parts of Candida bacteria, 1.6 parts of Alcaligenes faecalis, 0.8 parts of Achromobacter xylose oxidizing, 2.0 parts of Vibrio fibrous, and 1.2 parts of Phosphorus spp.;
[0122] Example ...
Embodiment 3
[0125] Composite microbial preparation, which comprises
[0126] Microorganism, same as Example 2;
[0127] Example 1 Citric acid modified fiber iron (III) complex (CAF-Fe).
[0128] The mass ratio of microorganisms and citric acid-modified fibrous iron(Ⅲ) complexes was 1:1.
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