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Method for improving induction rate of embryogenic calluses of pinus koraiensis

A technology for embryogenic callus and callus induction, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve research obstacles, low embryogenic callus induction rate, and physiological state requirements of explants Advanced problems, to achieve the effect of increasing the induction rate and improving the induction rate

Inactive Publication Date: 2021-06-11
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current somatic embryogenesis system of Korean pine still has the problems of low induction rate of embryogenic callus and high requirement of callus on the physiological state of explants (only explants at the proembryo stage can induce embryogenic callus) If you miss the appropriate physiological period, you will have to wait until the next year, so the research on the somatic embryogenesis system of Korean pine has limitations and obstacles

Method used

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  • Method for improving induction rate of embryogenic calluses of pinus koraiensis
  • Method for improving induction rate of embryogenic calluses of pinus koraiensis
  • Method for improving induction rate of embryogenic calluses of pinus koraiensis

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specific Embodiment approach 1

[0010] Specific embodiment one: a kind of method for improving the embryogenic callus induction rate of Korean pine in the present embodiment is carried out according to the following steps:

[0011] 1. Cultivate the Korean pine cones after pretreatment at a temperature of 4-6°C for 28-35 days to obtain the cultivated Korean pine cones; wash the cultivated Korean pine cones and peel off the scales, and then Carry out disinfection and washing treatment, then peel off the seed coat, continue disinfection and washing treatment to obtain sterilized female gametophytes; inoculate the sterilized female gametophytes in callus induction medium, and incubate at 21-25°C Under temperature conditions, culture in dark for 30-60 days to obtain cultured embryogenic callus;

[0012] Two, the embryogenic callus after the culture is mixed with the basal medium, shakes and centrifuges, and then filters to obtain the dispersed embryogenic cell mass, the quality of the embryogenic callus after the...

specific Embodiment approach 2

[0015] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the pretreatment of Korean pine cones in step one is to use 75% ethanol solution to evenly spray.

[0016] Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0017] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that in step one, the cultivated Korean pine cones are cleaned and peeled off the fruit scales, and then the steps of disinfecting and rinsing are: first use 70 Treat with ~75% ethanol solution for 1 ~ 2 minutes, then rinse with sterile water for 3 to 5 times, then treat with 10% sodium hypochlorite solution for 15 minutes, and continue to rinse with sterile water for 3 to 5 times.

[0018] Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention relates to a method for improving the induction rate of embryogenic calluses of pinus koraiensis, in particular to the method for improving the induction rate of the embryogenic calluses of the pinus koraiensis. The invention aims to solve the problem of low induction rate of the embryogenic calluses of the pinus koraiensis at present. The method comprises the following steps of pretreating pinus koraiensis cones, and culturing the pinus koraiensis cones at 4 to 6 DEG C for 28 to 35 days to obtain cultured pinus koraiensis cones; treating the cultured pinus koraiensis cones to obtain sterilized female gametophytes, then inoculating the sterilized female gametophytes into a callus induction culture medium, and performing dark culture at the temperature of 21 to 25 DEG C for 30 to 60 days to obtain cultured embryogenic calluses; mixing the cultured embryogenic calluses with a basic culture medium, oscillating and centrifuging, and filtering to obtain a dispersed embryogenic cell cluster; and placing the dispersed embryogenic cell cluster on a mature culture medium, and performing dark culture for 40 to 60 days to obtain a somatic embryo. The method for improving the induction rate of the embryogenic calluses of the pinus koraiensis can be obtained by the invention.

Description

technical field [0001] The invention relates to a method for improving the induction rate of Korean pine embryogenic callus. Background technique [0002] As a unique tree species in Northeast China, Korean pine not only has ecological value, but also has economic value. In recent years, the number of natural Korean pine forests has declined sharply due to unreasonable logging, so it is urgent to develop a breeding technology that can efficiently breed improved varieties. At present, the seed propagation cycle of Korean pine is long, the genetic variation is large, and the cutting propagation efficiency is low. Therefore, the somatic cell embryogenesis technology is currently considered to be the most potential large-scale rapid propagation technology of improved varieties, especially in coniferous trees. At the same time, the somatic embryogenesis technology can be combined with the controlled pollination and seed production among optimal clone individuals and the ultra-lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 彭春雪杨玲沈海龙
Owner NORTHEAST FORESTRY UNIVERSITY
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