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High-purity duck intramuscular precursor fat cell separation method

A technology of adipocytes and separation methods, which is applied to the separation of preadipocytes in meat duck muscle, the isolation of high-purity preadipocytes in duck muscle, culture and induced differentiation, which can solve the problem of reducing the economic value of carcass and affecting feed conversion Efficiency and other issues

Pending Publication Date: 2021-06-08
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Within a certain range, with the increase of fat content, the muscle quality will continue to improve; however, excessive deposition of abdominal and subcutaneous fat in poultry will not only affect the conversion efficiency of feed, but also reduce the economic value of the carcass

Method used

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  • High-purity duck intramuscular precursor fat cell separation method
  • High-purity duck intramuscular precursor fat cell separation method
  • High-purity duck intramuscular precursor fat cell separation method

Examples

Experimental program
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Effect test

Embodiment

[0030] In this embodiment, the process of isolation, culture and induction of preadipocytes in meat duck muscle is as follows:

[0031] 1. Soak 8-day-old ducklings in bromogeramine solution. After 3 minutes, the carotid artery was bled to death, then soaked in 75% ethanol for 5 minutes, and the breast muscle tissue was collected and placed in a preheated 2% double-antibody solution. In PBS buffer, work on separating cells between cells.

[0032] 2. Soak the collected pectoral muscle tissue in 75% alcohol for 1 min, transfer to 2% double-antibody PBS buffer, wash 3 times, and remove the visible fascia with high-pressure scissors and tweezers. Transfer the tissue block to a vial and cut it into 1mm 2 Then transfer the shredded tissue pieces to a 50ml centrifuge tube, add 10 times the volume of 0.1% type Ⅰ and type Ⅱ collagenase mixture (the volume ratio of type Ⅰ and type Ⅱ collagenase is 1:1), and put Seal it with a parafilm, shake it at 37°C, and digest it for 1 hour. At thi...

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Abstract

The invention discloses a high-purity duck intramuscular precursor fat cell separation method, and belongs to the technical field of cytology. The method comprises the following steps of soaking a cherry valley duck, bloodletting and soaking, collecting a duck breast muscle tissue sample, cleaning the tissue, removing fascia, cutting the breast muscle into pieces for digestion, terminating digestion, obtaining cells, discarding supernatant, blowing, beating and uniformly mixing, culturing the breast muscle, and the like. A large number of intramuscular precursor fat cells can be rapidly obtained, the difficulty of obtaining the cells for a long time is broken through, the method has important theoretical value for researching poultry fat deposition and meat quality, and a foundation is laid for carrying out subsequent experimental research.

Description

technical field [0001] The invention belongs to the technical field of cytology and relates to a high-purity method for isolating pre-adipocytes in duck muscle, in particular to a method for isolating, cultivating and inducing differentiation of pre-adipocytes in duck muscle. Background technique [0002] Fat content is an important economic trait of meat ducks, and intramuscular fat content is closely related to meat tenderness, taste and juiciness. Within a certain range, with the increase of fat content, the muscle quality will continue to improve; however, the excessive deposition of abdominal and subcutaneous fat in poultry will not only affect the conversion efficiency of feed, but also reduce the economic value of carcass. Therefore, increasing intramuscular fat content and reducing abdominal and subcutaneous fat are important goals pursued by current breeders. [0003] The intramuscular fat of poultry is different from that of mammals. It cannot be observed with the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
CPCC12N5/0653C12N2509/10C12N2509/00
Inventor 王志秀梁文双田慧月严丹董丙强毕瑜林常国斌陈国宏
Owner YANGZHOU UNIV
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