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Comamonas strain for preventing and treating tomato fusarium wilt, biocontrol inoculant, preparation method of biocontrol inoculant and application of comamonas strain and biocontrol inoculant

A technology for Comamonas and tomato wilt, applied in biochemical equipment and methods, botany equipment and methods, microbial-based methods, etc., can solve the pollution of soil and water natural environment, pathogenic bacteria resistance, human Health hazards and other issues, to achieve the effect of simple production process, short cultivation time, and obvious antibacterial effect

Pending Publication Date: 2021-06-08
南京振旭生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the prevention and control of tomato wilt, the measures of high-temperature and stuffy sheds are not ideal, and the occurrence of continuous cropping obstacles is still serious; and the long-term and large-scale use of chemical agents will pollute the natural environment such as soil and water bodies, and pose a hazard to human health. Pathogenic bacteria develop drug resistance, thereby reducing control efficacy

Method used

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  • Comamonas strain for preventing and treating tomato fusarium wilt, biocontrol inoculant, preparation method of biocontrol inoculant and application of comamonas strain and biocontrol inoculant
  • Comamonas strain for preventing and treating tomato fusarium wilt, biocontrol inoculant, preparation method of biocontrol inoculant and application of comamonas strain and biocontrol inoculant
  • Comamonas strain for preventing and treating tomato fusarium wilt, biocontrol inoculant, preparation method of biocontrol inoculant and application of comamonas strain and biocontrol inoculant

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Example 1: Isolation, purification and identification of Comamonas hydrochloride Ca28

[0023] Comamonas hydrochloride Ca28 for preventing and treating tomato wilt involved in the present invention was isolated from wheat rhizosphere soil in Tongshan District, Xuzhou City in 2019;

[0024] Separation method: 5-point sampling method was used to collect the wheat rhizosphere soil in the field, put them into sterilized sealed plastic bags, and bring them back to the laboratory immediately; add 5 g of the soil samples into 45 mL of sterile water, and culture them in a shaker at 150 r / min After 1 hour, the suspension was filtered with sterile gauze and a 10-6-fold dilution was obtained by 10-fold serial dilution. Take 100 μL of the diluted solution and spread it evenly on the NA plate. After culturing in a constant temperature incubator at 28°C for 48 hours, pick out single colonies with different colors, glossiness, size and type, streak and purify the strains, and transfer...

Embodiment 2

[0028] Embodiment 2: the preparation of biocontrol agent

[0029] Using the isolated Comamonas hydrochloride Ca28 to prepare a biocontrol agent, specifically includes the following steps: adding Comamonas hydroflora Ca28 to the NB medium, shaking and culturing at 28°C and 180rpm for 20 hours, to obtain seed liquid, seed liquid The total concentration of live bacteria in the medium is about 10 6 CFU / mL; Inoculate the seed liquid with 1% inoculum in the basal salt medium, shake and culture at 30°C and 180rpm for 72 hours, and obtain a total concentration of viable bacteria of 1×10 9 CFU / mL finished biocontrol agents.

[0030] NB medium: beef extract 3g, sodium chloride 5g, peptone 10g, distilled water to 1L, adjust pH to 7.0, and in 1×10 5 Pa sterilization for 30min.

[0031] NA solid medium: beef extract 3g, sodium chloride 5g, peptone 10g, agar 18g, distilled water to 1L, adjust pH to 7.0, and in 1×10 5 Pa sterilization for 30min.

[0032] Basal salt medium: glucose 5g, a...

Embodiment 3

[0033] Embodiment 3: Plate confrontation test detects the control effect of biocontrol fungicides on tomato fusarium wilt

[0034] The biocontrol bacterium agent that embodiment 2 is made is diluted 5 times to obtain the total concentration of living bacteria to be 2 * 10 8CFU / mL biocontrol agent, using mycelial growth rate method, transfer tomato wilt fungus (Fusarium oxysporum f.sp. lycopersici Snyderet Hansen) to PDA plate, activate at 25°C for 96h, and then Use a puncher to prepare a bacterium cake with a diameter of 5mm, and transfer it to a solution containing 0.1mL of live bacteria with a total concentration of 2×10 8 The PDA plate of CFU / mL biocontrol agent was used as the treatment group, and the control group was directly transferred the tomato Fusarium wilt bacteria to the PDA plate. Both the treatment group and the control group were cultured at 25°C, and the colonies in the control group grew to about the diameter of the plate. When the 4 / 5 of the bacterium was m...

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Abstract

The invention relates to a comamonas strain for preventing and treating tomato fusarium wilt, the comamonas strain is named as comamonas Ca28, the preservation date is November 9, 2020, and the preservation number is CGMCC NO.21148. The comamonas Ca28 provided by the invention has the advantages of simple production process, short culture time and low cost, a biocontrol inoculant for preventing and treating the tomato fusarium wilt can be prepared from the comamonas strain by a simple fast and low-cost preparation method. Experiments prove that the biocontrol inoculant provided by the invention has the prevention effect of 74.25% on the tomato seedling fusarium wilt under a greenhouse pot culture condition and the prevention effect of 48.75% on the tomato fusarium wilt under a field condition through the single application of the biocontrol inoculant, when the biocontrol inoculant is combined with 10% difenoconazole at the half application amount, and the prevention effect reaches 81.17%, and is superior to the prevention effect (79.43%) of the single application of 10% difenoconazole at the total amount.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a Comamonas hydrochloride strain for preventing and treating tomato wilt, a biocontrol agent, a preparation method and application thereof. Background technique [0002] Tomato is a vegetable with high economic value. It can be eaten directly as a fruit or cooked as a vegetable, and is generally loved by people. According to statistics from the Ministry of Agriculture, my country's tomato output reached 55.94 million tons in 2015, ranking first in Asia and taking a leading position in the world. As the proportion of tomatoes in people's lives is increasing, in order to better meet people's needs, the planting area of ​​facility tomatoes continues to increase, resulting in more and more serious damage to tomatoes by various diseases and insect pests, especially soil-borne tomatoes Fusarium wilt is particularly serious. The general incidence of tomato wilt is 10% to...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCC12N1/20A01N63/20
Inventor 贾思振韩先旭张磊丁浩然牛冬冬郭威
Owner 南京振旭生物科技有限公司
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