Method for biodegradation of carbon black particles induced by metabolic enzyme and method for analyzing product thereof

A technology of biodegradation and degradation products, which is applied in the field of analysis of biodegradation and its products, can solve the problems of carbon black chemical inertness and difficult to be biodegraded, and achieve the goal of reducing the co-crystallization process, promoting desorption and ionization, and signal stability Effect

Active Publication Date: 2021-06-04
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the chemical properties of carbon black are relatively inert and difficult to be biodegraded.

Method used

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  • Method for biodegradation of carbon black particles induced by metabolic enzyme and method for analyzing product thereof
  • Method for biodegradation of carbon black particles induced by metabolic enzyme and method for analyzing product thereof
  • Method for biodegradation of carbon black particles induced by metabolic enzyme and method for analyzing product thereof

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Embodiment

[0072] Prepare an aqueous dispersion of carbon black particles at a certain concentration, mix the carbon black particles with liver metabolic enzymes (cytochrome P450, glutathione transferase), and then put them into a water-proof incubator for incubation. The mixture was cultured continuously for 14 days. To simulate the biological environment, these experiments were first performed in a dark environment at 37 °C. After finding that carbon black particles can be degraded by metabolic enzymes, the applicant also carried out light irradiation experiments to test the application potential of this technology in wastewater purification. The light intensity is maintained at 550W / m 2 , the solution is monitored at certain time intervals to monitor the conversion of carbon black particles. All experiments were repeated three times (n=3). This application uses the fly ash sample as an example of an actual sample here. The pretreatment process of the actual sample is as follows: f...

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Abstract

The invention discloses a method for biodegradation of carbon black particles induced by metabolic enzyme and a method for analyzing a product thereof. The method comprises the following steps: step (1), weighing a proper amount of carbon black sample; (2) dispersing the weighed carbon black sample with water and preparing a dispersion liquid with a certain concentration; (3) mixing the carbon black dispersion liquid with a metabolic enzyme solution, and uniformly mixing the mixed liquid in a vortex oscillator; and (4) placing the mixed solution in a waterproof incubator for incubation. The method disclosed by the invention reveals the action of the enzyme through complementary multiple technologies, and proves that the enzyme can induce the conversion of the carbon black particles. According to a typical carbon cluster mass spectrum peak and changes of carbon-oxygen and carbon-nitrogen bonds in a spectrum, a conversion mechanism of carbon black particles in a living body is provided.

Description

technical field [0001] The invention belongs to the field of biodegradation and its analysis, and more specifically relates to an analysis method based on the biodegradation of carbon black particles induced by metabolic enzymes and its products. Background technique [0002] Carbon black particles are the products of incomplete combustion or thermal decomposition of hydrocarbons. Because of their low cost, high conductivity, and small size, they are widely used as reinforcing fillers for filters, elastomers, plastics, paints, and tires, fuel cells and Conductive additives and catalyst supports in electrode materials. The large amount of these particles emitted into the environment also increases the risk of human exposure. A large number of epidemiological results show that carbon black has extremely strong toxicity and biological effects, and has a great impact on human health. Its toxicity is much higher than other fine particles. Carbon black has small particle size, ...

Claims

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Application Information

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IPC IPC(8): G01N27/623G01N27/624G01N1/38
CPCG01N27/622G01N1/38
Inventor 刘倩黄秀江桂斌
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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