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Application of CIT-H3 protein detection reagent in preparation of liver cancer prognosis and/or recurrence prediction reagent

A CIT-H3, 1. CIT-H3 technology, applied in the field of life sciences, can solve the problems of high cost and technical threshold, low detection sensitivity of diagnosis and targeted drug screening markers, etc., and achieve the effect of easy operation

Inactive Publication Date: 2021-06-01
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no specific marker detection scheme for patient prognosis and recurrence assessment in the clinical diagnosis and treatment of liver cancer. Commonly used marker detection for diagnosis and targeted drug screening have shortcomings such as low sensitivity and high cost and technical threshold.

Method used

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  • Application of CIT-H3 protein detection reagent in preparation of liver cancer prognosis and/or recurrence prediction reagent
  • Application of CIT-H3 protein detection reagent in preparation of liver cancer prognosis and/or recurrence prediction reagent
  • Application of CIT-H3 protein detection reagent in preparation of liver cancer prognosis and/or recurrence prediction reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Neutrophils produce "neutrophil extracellular traps" to promote malignant progression of liver cancer

[0028] 1. Experimental method

[0029] In this implementation case, neutrophils were first isolated from the peripheral blood of healthy people, cultured in vitro by adding tumor cell culture supernatant, and neutrophil extracellular traps were induced and purified. Then use the Transwell chamber experiment, add purified neutrophil extracellular traps, and detect the changes in the invasion ability of the human liver cancer cell line HepG2 cells in the Transwell chamber, so as to evaluate the effect of neutrophil extracellular traps on the malignant progression of tumor cells influences.

[0030] 2. Experimental results

[0031] The result is as figure 1 As shown, compared with the control group, after adding the "neutrophil extracellular trap", the number of human liver cancer cells invading to the lower surface of the Transwell chamber increased, and th...

Embodiment 2

[0032] Example 2 A liver cancer patient's poor and recurrence rate prediction kit

[0033] In this example, CIT-H3 antigen was selected as a marker for the production of "neutrophil extracellular traps" in tumor tissues, and a kit for detecting its production level was established by using traditional immunohistochemical staining.

[0034] 1. Composition

[0035] The primary antibody CIT-H3 (purchased from Abcam, product number is ab5103) and the second anti-rabbit IgG antibody labeled with horseradish peroxidase (purchased from Dako company, product number is P044801-2).

[0036] 2. How to use

[0037] 1. Specimen detection

[0038] (1) Select a paraffin section of liver cancer that includes the tumor area, and ensure that there is no large area of ​​necrosis;

[0039] (2) Obtain 8 paraffin sections of 4 μm, bake the slices at 60°C for 2 hours, take them out, and cool slightly;

[0040] (3) Dewaxing with xylene twice at room temperature, 10 minutes each time;

[0041] (4...

Embodiment 3

[0064] Example 3 The specific expression of CIT-H3 "neutrophil extracellular trap" is associated with poor prognosis and high recurrence rate of patients

[0065] 1. Experimental method

[0066] In this implementation case, 80 cases of liver cancer patients with surgically resected liver cancer samples and relevant clinical pathological information were collected, and the expression of CIT-H3 in the liver cancer tissues of these patients was detected by using the kit in implementation 2. After statistical analysis, combined with the prognosis and survival of the patients The data were analyzed by COX risk regression, and combined with pathological information such as recurrence, the chi-square correlation test was performed.

[0067] 2. Experimental results

[0068] The results showed that grouping by using the expression level of CIT-H3 can effectively predict the overall survival and recurrence time of patients, and there is a significant difference. (Overall survival P=0....

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Abstract

The invention discloses an application of a CIT-H3 protein detection reagent in preparation of a liver cancer prognosis and / or recurrence prediction reagent, and provides an immunohistochemical detection kit for evaluating the prognosis and recurrence risk of a liver cancer patient. According to the principle of immunohistochemistry, the kit detects a marker CIT-H3 (citrullinated histone H3) of a neutrophile granulocyte extracellular trap net, has the characteristics of being objective, accurate, simple, convenient, intuitive, easy to operate and the like, and can be operated by common technicians in a hospital pathology room without the help of complex instruments; the prognosis and recurrence risk of the liver cancer patient can be effectively evaluated, so that a doctor is better helped to monitor the condition of the patient, proper treatment means are adjusted in time, the treatment effect is improved, and the survival time of the patient is prolonged.

Description

technical field [0001] The invention relates to the technical field of life sciences, in particular to the application of a CIT-H3 protein detection reagent in the preparation of liver cancer prognosis and / or recurrence prediction reagents. Background technique [0002] Primary hepatocellular carcinoma (hepatocellular carcinoma for short) is one of the most common malignant tumors in my country, and its morbidity and mortality rank the fifth and second respectively among malignant tumors in my country. At present, the most commonly used treatment methods for liver cancer are still surgical resection and liver transplantation. However, liver cancer is highly malignant, and has the characteristics of rapid development and easy metastasis, which leads to poor prognosis and rapid overall recurrence of liver cancer patients, making the overall five-year survival rate of liver cancer still at a low level. Therefore, by finding relevant markers to evaluate the prognosis and recurr...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/68G01N33/57438G01N33/57484G01N2333/47
Inventor 郑利民蒋泽洲吴艳
Owner SUN YAT SEN UNIV
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