Mesenchymal stem cell preparation for treating knee osteoarthritis and research method thereof
A technology of knee osteoarthritis and stem cells, applied in the field of chemical medicine, can solve the problems of limited quantity and low immunogenicity
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[0016] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0017] see figure 1 , a mesenchymal stem cell preparation for treating knee osteoarthritis and a research method thereof, comprising the following steps:
[0018] Step A: Prepare the platelet lysate, take 300ml of venous blood from the patient, centrifuge to obtain the platelet lysate, store it at -80°C for later use;
[0019] Step B: Isolation and culture of human umbilical cord MSCs, rinse the umbilical cord with sterile saline in an ultra-clean bench, and ...
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