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Fibrous protein adhesive quick-acting hemostatic composite powder and preparation method thereof

A technology of fibrin and fibrinogen, applied in surgical adhesives, pharmaceutical formulations, applications, etc., can solve the problems of inconvenient clinical use, complicated preparation process, high storage and transportation costs, and achieve simple clinical application, simple preparation process, Significant Technical Effects

Active Publication Date: 2021-05-28
SHANGHAI LIKANGRUI BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve a series of technical problems such as complex preparation process of existing fibrin adhesive products, inconvenient clinical use, high storage and transportation costs, the present invention provides a fibrin adhesive fast-acting hemostatic composite powder and its preparation method

Method used

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  • Fibrous protein adhesive quick-acting hemostatic composite powder and preparation method thereof
  • Fibrous protein adhesive quick-acting hemostatic composite powder and preparation method thereof

Examples

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Effect test

Embodiment 1

[0032] A preparation method of fibrin adhesive fast-acting hemostatic composite powder, comprising the steps of:

[0033] (1) Preparation of refined fibrinogen precipitate: Frozen plasma was melted in a water bath at 37° C., tributyl phosphate and polysorbate 80 were added at a ratio of 1% to a final concentration, mixed evenly, and stirred and incubated at 25° C. for 6 hours. Cool the plasma down to 1°C, add -15°C, 50% ethanol solution according to the final ethanol concentration of 10%, alcohol precipitation at 1°C for 6 hours, and collect the precipitate by centrifugation to obtain a fibrinogen precipitate. Crush the fibrinogen precipitate once in 10% ethanol solution at 1°C, stir for 30 min, and collect the precipitate by centrifugation. Pulverize the precipitate in a complex solution (0.85% sodium citrate, 0.65% sodium chloride, pH 7.0), and redissolve at 37°C for 60 minutes to obtain a primary complex solution. The temperature of the primary complex solution was lowered...

Embodiment 2

[0039] A preparation method of fibrin adhesive fast-acting hemostatic composite powder, comprising the steps of:

[0040] (1) Preparation of refined fibrinogen precipitate: Frozen plasma was melted in a water bath at 37° C., tributyl phosphate and polysorbate 80 were added at a ratio of 1% to a final concentration, mixed evenly, and stirred and incubated at 25° C. for 6 hours. Cool the plasma down to 1°C, add -15°C, 50% ethanol solution according to the final ethanol concentration of 10%, alcohol precipitation at 1°C for 6 hours, and collect the precipitate by centrifugation to obtain a fibrinogen precipitate. Crush the fibrinogen precipitate once in 10% ethanol solution at 1°C, stir for 30 min, and collect the precipitate by centrifugation. Pulverize the precipitate in a complex solution (0.85% sodium citrate, 0.65% sodium chloride, pH 7.0), and redissolve at 37°C for 60 minutes to obtain a primary complex solution. The temperature of the primary complex solution was lowered...

Embodiment 3

[0046] A preparation method of fibrin adhesive fast-acting hemostatic composite powder, comprising the steps of:

[0047] (1) Preparation of refined fibrinogen precipitate: Frozen plasma was melted in a water bath at 37°C, tributyl phosphate was added at a ratio of 0.3% and polysorbate 80 was added at a ratio of 1% at a final concentration, mixed evenly, stirred and incubated at 25°C for 6 hours. Cool the plasma down to 1°C, add -15°C, 50% ethanol solution according to the final ethanol concentration of 10%, alcohol precipitation at 1°C for 1 hour, and centrifuge to collect the precipitate to obtain a fibrinogen precipitate. Crush the fibrinogen precipitate once in 10% ethanol solution at 1°C, stir for 30 min, and collect the precipitate by centrifugation. Pulverize the precipitate in a complex solution (0.85% sodium citrate, 0.65% sodium chloride, pH 7.0), and redissolve at 37°C for 60 minutes to obtain a primary complex solution. The temperature of the primary complex solut...

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Abstract

The invention belongs to the technical field of biomedical materials, and particularly relates to fibrous protein adhesive quick-acting hemostatic composite powder and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a fibrous protein suspension; (2) preparing a thrombin suspension; and (3) fully mixing the prepared fibrous protein suspension and thrombin suspension, filling, drying in vacuum, and performing radiation sterilization. The fibrous protein adhesive quick-acting hemostatic composite powder is simple in preparation process, does not need to be prepared in clinical application, can be used after being opened, can be stored and transported at room temperature, has remarkable technical, cost and clinical advantages, and has wide clinical application and market prospects.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a fibrin adhesive fast-acting hemostatic composite powder and a preparation method thereof. Background technique [0002] Difficulty suturing clinical wound bleeding and postoperative suture oozing often bring troubles to the completion of surgery and postoperative recovery of patients. Choosing a suitable medical adhesive can effectively solve this problem. Fibrin adhesive has good hemostasis, tissue compatibility and adhesiveness. By simulating the terminal stage of blood coagulation, fibrinogen is cracked into fibrin monomer under the action of thrombin, and at the same time, under the action of calcium Coagulation factor XIII generates active coagulation factors, and fibrin monomers are cross-linked under the catalysis of active coagulation factors to form viscous gel-like fibrin clots to achieve the effect of hemostasis and adhesion. [0003] At present, the ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/00
CPCA61L24/106A61L24/001A61L24/108A61L24/0015A61L2400/04A61L2300/254
Inventor 刘璐张理火张军东吴剑英
Owner SHANGHAI LIKANGRUI BIOLOGICAL ENG
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