Parthenocarpy breeding method of industrial hemp and application of parthenocarpy breeding method
An industrial hemp, parthenocarpic technology, applied in the field of breeding, which can solve problems such as lack of industrial hemp varieties
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[0054]The following will be appreciated by the following examples, and the following examples are intended to illustrate the invention.
[0055]The instruments, equipment and agents used in the examples can be obtained by various channels, such as purchasing, or can be prepared.
[0056]Early identification of marijuana female strains: uses the specific SCAR molecular molecular markers of the cannabis strains, by simple PCR analysis, can quickly and effectively identify cannabis female strains.
[0057]Sampling seedlings or unflowing plants, using a DNA rapid extraction method based on SDS (SODIUM DODECYL SULPHATE) to extract genomic DNA, and the specific SCAR tag PCR detection identification of the cannabis strain is performed (MandolinoTal., 1999). The PCR reaction system uses the Quick TAQ HS DYEMIX (DTM-101) kit (DTM-101) kit, 20 microliters of PCR reaction system comprising: 10 μL 2X Quick TAQ HS Dyemix, 1.0 μL 10 pmol / μL primer MADC2-F (gtgacgtaggatagtgaa (SEQ ID NO: 1)) or MADC5-F (ga...
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