Fungus community structure regulation and control method for reducing damage of pathogenic bacteria of shoot blight to a host plant
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A technology of host plants and community structure, applied in botany equipment and methods, plant growth regulators, plant growth regulators, etc.
Active Publication Date: 2021-05-25
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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[0005] So far, there has been no report on the biological control of reducing the damage of the pathogen of blight blight to host plants by regulating the structure of the host plant's endophytic microbial community
Method used
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Embodiment 1
[0031] Example 1 Regulation of pine dieback based on endophytic fungal community structure
[0032] (1) Host plant inoculation
[0033] Inoculate Jattaea taediosa and Aureobasidium pullulans bacteria or spray J.taediosa and A.pullulans fermentation broth on the red pine in the forest plot of Kunyu Mountains, and directly spray the fermentation broth to the tips and branches to increase the relative abundance and inhibit pine Growth and reproduction of S. sapinea.
[0034] (2) Cleaning and pruning of diseased branches
[0035] Remove the diseased branches infected with dieback in time to prevent the further spread of pathogenic bacteria. For vigorously growing forest stands, pruning or thinning of "retaining the good and removing the bad" should be adopted in time to slow down the ineffective transpiration and enhance the tree vigor.
Embodiment 2
[0036] Example 2 Regulation of pine dieback based on endophytic fungal community structure
[0037] Concrete method is with embodiment 1, and difference is, to the red pine spraying Orbiliarectispora in Kunyu Mountain inter-forest plot, Phaeothecoidea sp., Devriesia sp., Cryptococcus sp., the antagonistic antibiotics of bacteria such as Orbilia sp., above-mentioned fungus mostly is The pathogenic bacteria of common diseases can simultaneously inhibit the growth of S. sapinea by antagonizing the above-mentioned pathogenic bacteria.
Embodiment 3
[0038] Example 3 Regulation of pine dieback based on endophytic fungal community structure
[0039]The specific method is the same as in Example 1, the difference is that Jattaeataediosa, Aureobasidium pullulans bacterium or spraying J.taediosa, A.pullulans fermentation broth are inoculated to the red pine in the forest plot of Kunyu Mountains, and the antagonistic Orbilia rectispora, Phaeothecoidea sp., Devriesia are sprayed sp., Cryptococcus sp., Orbilia sp. and other bacterial antagonists, increase the relative abundance of J.taediosa, A.pullulans, and inhibit the growth of a variety of pathogenic bacteria.
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Abstract
The invention discloses a fungus community structure regulation and control method for reducing damage of pathogenic bacteria of shoot blight to a host plant. The fungus structure regulation and control method comprises the steps that an endophytic fungus community structure of the host plant is regulated, and the mode of adjusting the endophytic fungus community structure of the host plant comprises the step of improving the relative abundance of endophytic fungi which are negatively related to the pathogenic bacteria of the shoot blight or reducing the relative abundance of endophytic fungi which are positively related to the pathogenic bacteria of the shoot blight; or the relative abundance of the endophytic fungi positively related with the pathogenic bacteria of the shoot blight is reduced while the relative abundance of the endophytic fungi negatively related with the pathogenic bacteria of the shoot blight is improved. According to the fungus community structure regulation and control method for reducing the damage of the pathogenic bacteria of the shoot blight to the host plant, by adjusting the structure of an endophytic fungus community of Pinus densiflora, the fungus community is transformed into a structure which is not beneficial to survival of the pathogenic bacteria of the shoot blight, so that the pathogenic bacteria are difficult to proliferate and diffuse, the harm to the Pinus densiflora forest is effectively reduced, and the ecological environment of the distribution area of the Pinus densiflora forest is maintained and protected.
Description
technical field [0001] The invention relates to a method for preventing and treating pine dieback disease, in particular to a method for reducing damage to host plants by endophytic fungal community structure, and belongs to the field of biological control of pine dieback disease. Background technique [0002] Pine dieback is one of the most common and widely distributed important diseases on conifers in the world. The disease mainly manifests as dead terminal buds, dead needles, dead tips or bushes, etc., and can also cause trunk ulcers on some hosts. , fat flow, necrosis, and root rot and wood blue stain, etc., young trees and large trees can be affected. Pine dieback has caused serious damage and loss to pine plantations in many countries in the world, and has aroused the general concern of forestry workers all over the world. [0003] The damage caused by Sphaeropsis sapinea has the characteristics of wide incidence, severe damage, and long disease period. It is distrib...
Claims
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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00A01N63/30A01P21/00
CPCA01G13/00
Inventor 梁军谢宪胡瑞瑞张星耀
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY