Production-type two-dimensional liquid chromatography interface and production-type two-dimensional liquid chromatography separation system

Abstract

The invention provides a production-type two-dimensional liquid chromatography interface and a production-type two-dimensional liquid chromatography separation system. The production-type two-dimensional liquid chromatography interface comprises a production-type two-dimensional liquid chromatography interface, a first-dimensional liquid phase system and a second-dimensional liquid phase system. Through the production type two-dimensional liquid chromatogram interface based on the enrichment column array, the problems of synchronous conflict and control of first-dimensional liquid chromatogram separation and second-dimensional liquid chromatogram separation are solved, and continuous and efficient operation of production type two-dimensional liquid chromatogram separation can be realized.

Description

technical field [0001] The invention belongs to the technical field of high-performance liquid chromatography separation, and relates to a production-type two-dimensional liquid chromatography interface and a production-type two-dimensional liquid chromatography separation system. Background technique [0002] The operating modes of the online two-dimensional liquid chromatography system are divided into single heart-cutting, multiple heart-cutting and continuous cutting. In the process of two-dimensional chromatographic separation in multiple heart-cutting mode and continuous cutting mode, it is necessary to ensure that the two-dimensional separation is carried out simultaneously. In order to keep the first and second dimensions running synchronously, the gradient time of the second dimension must be smaller than the cutting time of the first dimension. For this reason, higher flow rates are required in the second dimension. Although a higher flow rate can further increas...

AI Technical Summary

Problems solved by technology

The injection volume of the analytical two-dimensional liquid chromatography system is very small. In order to realize the synchronization of the two-dimensional separation, the co-eluted chromatographic peak fractions are cut multiple times, resulting in a small amount, trace level or trace amount of the two-dimensional liquid chromatography separation. Level of repeated samples, unable to complete large-scale sample preparation

Using two enrichment columns to enrich and separate alternately, although some two-dimensional preparative chromatography systems can realize two-dimensional liquid phase preparation, the development time of the preparation method is long, and the synchronization problem of two-dimensional separation has not been completely solved

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