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Biomarker for early detection of severe hepatitis B virus as well as detection kit and application thereof

A technology for biomarkers and early detection, applied in the field of biomarkers and detection kits for early detection of severe hepatitis B virus

Pending Publication Date: 2021-05-14
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no effective method and means to evaluate the effect of clinical treatment in clinical practice.

Method used

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  • Biomarker for early detection of severe hepatitis B virus as well as detection kit and application thereof
  • Biomarker for early detection of severe hepatitis B virus as well as detection kit and application thereof
  • Biomarker for early detection of severe hepatitis B virus as well as detection kit and application thereof

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Experimental program
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Embodiment 1

[0038] The invention mainly collects the peripheral venous blood of 77 cases of severe hepatitis B patients in Qilu Hospital of Shandong University.

[0039] Diagnostic criteria for severe hepatitis B: ① chronic hepatitis B history, HBsAg positive > 6 months; ② sudden severe jaundice with bilirubin rise ≥ 85mol / L and INR ≥ 1.5 (or PTA ≤ 40%); ③ ascites or hepatic encephalopathy.

[0040] Diagnostic criteria for chronic hepatitis B: ① HBsAg positive > 6 months; ② Serum HBV DNA > 20000 IU / mL (105 copies / mL), or 2000-20000 IU / mL (104-105 copies / mL) (common in HBeAg-negative CHB patients ); ③ Persistent or intermittent elevated ALT / AST levels; ④ Liver biopsy showed chronic liver inflammation with moderate or severe necrosis;

[0041] Exclusion criteria: ① combined with other hepatitis virus infection (hepatitis A virus, hepatitis C virus, hepatitis D virus, hepatitis E virus, etc.) and / or human immunodeficiency virus; ② suffering from other liver diseases, such as autoimmune hepa...

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Abstract

The invention relates to the technical field of biomedicine, in particular to a biomarker for early detection of severe hepatitis B virus as well as a detection kit and application thereof. The invention provides a biomarker for early detection of severe hepatitis B virus. The sequence of the biomarker is as shown in SEQ ID NO. 1. The invention further provides application of the biomarker in preparation of a kit for early detection of the severe hepatitis B virus and the kit for early detection of the severe hepatitis B. The kit comprises a primer pair and a probe for specific detection of GSTP1 methylation, the primer pair and the probe are used for detecting the reference gene ALU-C4. The detection method disclosed by the invention can be used for detecting trace DNA in peripheral blood of a human body, is high in sensitivity and strong in specificity, and is widely applied to detection of specimens with low DNA content and high loss rate.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a biomarker for early detection of severe hepatitis B virus, a detection kit and application thereof. Background technique [0002] Severe hepatitis is a serious liver disease characterized by a large number of liver cell necrosis on the basis of chronic liver disease or cirrhosis. Its main manifestations are jaundice, coagulation dysfunction, hepatic encephalopathy and ascites, etc., with a high incidence of complications , is one of the leading causes of death in patients with liver disease. my country is a high prevalence area of ​​hepatitis B, and hepatitis B virus infection is the main cause of severe hepatitis in my country. Severe hepatitis B is in a dangerous condition, with poor prognosis and high fatality rate. At present, there is no effective method and means to evaluate the effect of clinical treatment in clinical practice. Therefore, there is an urgent need t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6851C12N15/11
CPCC12Q1/6883C12Q1/6851C12Q2600/154C12Q2600/166C12Q2600/118
Inventor 王凯王婧雯向琳范玉琛高帅
Owner SHANDONG UNIV
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