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Peony stamen extract and its preparation method and application

A technology of peony stamens and extracts, which is applied in the field of peony stamens extracts and its preparation, can solve the problems of lack of analysis of the medicinal value of extracts, and achieve the effects of saving costs, increasing safety, and simplifying the process

Active Publication Date: 2022-01-28
INST OF BOTANY CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The peony stamen products or preparations disclosed above are mainly based on the traditional understanding and efficacy of peony stamens. The main product is stamen tea, and the preparation method is mainly simple drying and water extraction, and there is no analysis of the medicinal value of the extract.

Method used

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  • Peony stamen extract and its preparation method and application
  • Peony stamen extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, prepare peony stamen extract A

[0033] One, the preparation method of peony stamen extract A comprises the following steps:

[0034] (1) drying and pulverizing peony stamens to obtain dried peony stamens powder;

[0035] (2) According to the solid-liquid ratio of 1g: 15ml, add ethanol-water solution with a volume percentage concentration of 50% to the dry peony stamen powder obtained in step (1), and ultrasonically extract 3 times at 22°C, each time for 30 minutes , 15 minutes, and 15 minutes, collect and combine the extracts, concentrate the obtained extracts under reduced pressure until there is no alcohol smell, and obtain an aqueous solution of the ethanol extract of peony flower;

[0036] (3) Dilute the aqueous solution of peony flower alcohol extract obtained in step (2) with water until the relative density is 1.10, add ethyl acetate:water in a volume ratio of 1:0.8 and add water-saturated ethyl acetate solution for extraction, at 22°C Extracting...

Embodiment 2

[0049] Embodiment 2, preparation of peony stamen extract B

[0050] One, the preparation method of peony stamen extract B comprises the following steps:

[0051] (1) drying and pulverizing peony stamens to obtain dried peony stamens powder;

[0052] (2) According to the solid-liquid ratio of 1g: 12ml, add ethanol-water solution with a volume percentage concentration of 60% to the dried peony stamen powder obtained in step (1), and ultrasonically extract 2 times at 25°C, each time for 30 minutes , 15 minutes, collect the combined extracts, concentrate the obtained extracts under reduced pressure to no alcohol smell, and obtain an aqueous solution of the ethanol extract of Paeoniae peony;

[0053] (3) Dilute the aqueous solution of peony flower alcohol extract obtained in step (2) with water until the relative density is 1.20, add ethyl acetate:water in a volume ratio of 1:1 and add water-saturated ethyl acetate solution for extraction, at 25°C Extracting 3 times, combining th...

Embodiment 3

[0061] Embodiment 3, prepare peony stamen extract C

[0062] One, the preparation method of peony stamen extract C comprises the following steps:

[0063] (1) drying and pulverizing peony stamens to obtain dried peony stamens powder;

[0064] (2) According to the solid-liquid ratio of 1g: 15ml, add ethanol-water solution with a volume percentage concentration of 40% to the dried peony stamen powder obtained in step (1), ultrasonically extract once at 27°C for 30 minutes, and collect the extract , concentrating the obtained extract under reduced pressure until there is no alcohol smell, and obtaining an aqueous solution of the ethanol extract of peony flower;

[0065] (3) Dilute the aqueous solution of peony flower ethanol extract obtained in step (2) with water until the relative density is 1.10, add ethyl acetate:water in a volume ratio of 1:1.5 and add water-saturated ethyl acetate solution for extraction, at 27°C Extracting 3 times, combining the extracts, concentrating u...

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Abstract

The invention discloses a peony stamen extract, a preparation method and application thereof. The preparation method of the peony stamen extract provided by the present invention comprises the following steps: (1) drying and pulverizing the peony stamens to obtain dried peony stamens powder; (2) ultrasonically extracting the peony stamens powder with an aqueous alcohol solution , collecting and merging the extracts to obtain an aqueous solution of the ethanol extract of peony; (3) adding water to the aqueous solution of the ethanol extract of peony obtained in step (2), and extracting with ethyl acetate to obtain the ethyl acetate extract of peony; ( 4) Purify the ethyl acetate extract of peony stamens obtained in step (3) with a D101 macroporous adsorption resin column, collect the eluate, concentrate to extract state, and obtain the target extract of peony stamens.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to peony stamen extract and its preparation method and application. Background technique [0002] Peony (Paeoniasuffruticosa Andr.) is a plant of Paeoniaceae (Paeoniaceae) and the genus Paeonia (PaeoniaL.), which is a perennial deciduous shrub. Peony is not only a rare woody flower unique to China, with thousands of years of natural growth and more than 2,000 years of artificial cultivation history, but also a traditional medicinal and edible plant resource. Peony stamen is a potential functional food. Peony stamens are rich in carbohydrates, proteins, unsaturated fatty acids and minerals, which can supplement the nutrients needed by the human body. [0003] At present, most of the relevant research on peony stamens is in the field of food. CN103190504B discloses a processing method of peony stamen tea, which includes the following steps: raw material selection, drying in the air, dr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/71A61K36/65A61P35/00A61P15/14A61P3/04A61K8/9789A61Q19/06A23L33/105A61K133/00
CPCA61K36/71A61K36/65A61P35/00A61P15/14A61P3/04A61K8/9789A61Q19/06A23L33/105A61K2236/333A61K2236/39A61K2236/55A61K2800/805A61K2800/82A23V2002/00A23V2200/332A23V2200/308A23V2250/21A23V2300/14A23V2300/30A23V2300/38
Inventor 舒庆艳马晓丰方令豪
Owner INST OF BOTANY CHINESE ACAD OF SCI
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