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Application of small molecule compound in preparation of medicine for preventing or treating pebrine

A technology of small molecular compound and microparticle disease, applied in the fields of molecular biology and pathology, can solve the problems of ineffective effect of silkworm microparticle disease and no significant improvement in the incidence rate, and achieve the effect of reducing the incidence rate and inhibiting replication.

Active Publication Date: 2021-05-14
ZHENJIANG COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] The study on the chemical treatment of silkworm micronosis has been tried for a long time. It was found that fumagillin and anisomycin have inhibitory effects on the proliferation of the bee micronose protozoan Nosema apis, and benlet has an effect on the alfalfa weevil micron protozoa Nosema apis. The proliferation of sp. can be significantly inhibited, but the effect of these drugs on silkworm microparticle disease is not obvious
At present, the chemotherapeutic drugs used in production in China mainly include Fangweiling, Yuanzhangjing and Yuweiling, but their effects on silkworm microparticle disease are mainly reflected in the prevention. Adding drugs to silkworms infected with No. spp. There was no significant improvement in disease incidence, so it is necessary to study treatments that have beneficial effects on diseased silkworms infected with Microsporidia

Method used

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  • Application of small molecule compound in preparation of medicine for preventing or treating pebrine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Inoculation of cells and preparation of storage solutions for small molecules

[0022] (1) The silkworm ovary cell line BmN is cultured in TC100 insect cell culture medium containing 10% FBS (fetal bovine serum);

[0023] (2) Preparation of storage solutions for small molecules

[0024] DMSO was used to prepare a stock solution of small molecule compounds with a concentration of 10 mM;

[0025] (3) In vitro germination and infection of Bombyx mori Nos.

[0026] Use 1ml of 0.1mol / L KOH solution to resuspend the spores of N. silkworm, and incubate at 30°C for 30 minutes, then fully mix with 12ml of cell suspension; after standing for 5 minutes, inject an equal amount into a 12-well cell culture plate, and incubate at 28°C After standing for 1 hour to make it completely adhere to the wall, remove the culture medium, add fresh culture medium and seal the culture; change the fresh culture medium every 3 days, lyse the cells after 12 days, and count the microsporidia of ...

Embodiment 2

[0030] When the final concentration of small molecules is 0.1μM, the prevention and treatment of No. silkworm infection in cells:

[0031] Resuspend the refined N. silkworm spores with 1ml 0.1mol / L KOH solution, incubate at 30°C for 30 minutes, and then fully mix with 12ml of cell suspension; after standing for 5 minutes, inject an equal amount into a 12-well cell culture plate, After standing at 28°C for 1 hour to make it completely adhere to the wall, remove the medium, add fresh medium containing a final concentration of 0.1 μM small molecule compound, and seal the culture. The fresh medium (containing small molecule compounds) was changed every 3 days, and the cells were lysed after 12 days, and the microsporidia of Bombyx mori were counted with a hemocytometer. The measured silkworm Microsporidia spore titer is 356000pfu / mL (as figure 1 ).

Embodiment 3

[0033] When the final concentration of small molecule is 0.5 μ M, to the prevention and treatment of Bombyx mori infection in the cell, concrete steps are as follows:

[0034] Resuspend the refined N. silkworm spores in 1ml, 0.1mol / L KOH solution, incubate at 30°C for 30 minutes, and then fully mix with 12ml of cell suspension; after standing for 5 minutes, inject an equal amount into a 12-well cell culture plate After standing at 28° C. for 1 h to make it completely adhere to the wall, the medium was removed, and fresh medium containing a small molecule compound at a final concentration of 0.5 μM was added again, and cultured in a sealed manner. The fresh medium (containing small molecule compounds) was changed every 3 days, and the cells were lysed after 12 days, and the microsporidia of Bombyx mori were counted with a hemocytometer. The measured spore titer of N. silkworm was 0, that is, no mature spores were observed ( figure 1 ).

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Abstract

The invention provides an application of a small molecule compound in preparation of a medicine for preventing or treating pebrine. The structure of the small molecule compound is as shown in a general formula (I) aiming at core protein of nosema bombycis, and the three-dimensional structure of the small molecule compound is obtained by utilizing a virtual modeling technology based on a mainstream molecular docking technology. The small molecule compound can be used for preparing a medicine for preventing and treating pebrine, when the final use concentration of the small molecule compound in cells is 0.5 [mu]M or above, replication of nosema bombycis can be inhibited, and the morbidity of pebrine can be remarkably reduced when the small molecule compound is added to silkworms with the concentration of 1 mM or above. The method can be used for research, development and production of medicines for preventing and treating pebrine, and has application and popularization values.

Description

technical field [0001] The invention relates to the fields of molecular biology and pathology, and relates to the application of a small molecule compound in the preparation of a drug for preventing or treating silkworm micronosis. Background technique [0002] Silkworm microparticle disease is an ancient and devastating infectious disease of silkworms produced by Nosema bombycis orally infecting silkworms. . [0003] The study on the chemical treatment of silkworm micronosis has been tried for a long time. It was found that fumagillin and anisomycin have inhibitory effects on the proliferation of the bee micronose protozoan Nosema apis, and benlet has an effect on the alfalfa weevil micron protozoa Nosema apis. The proliferation of sp. can be significantly inhibited, but the effect of these drugs on silkworm microparticle disease is not obvious. At present, the chemotherapeutic drugs used in production in China mainly include Fangweiling, Yuanzhangjing and Yuweiling, but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/404A61P33/02
CPCA61K31/404A61P33/02
Inventor 吕丁丁唐旭东杨军万爱军
Owner ZHENJIANG COLLEGE
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