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A cell membrane-coated au-fe 3 o 4 Targeted nanomaterials and their preparation methods and applications

An au-fe3o4 and nanomaterial technology, which is applied in the field of targeted nanomaterials coated with Au-Fe3O4 on cell membranes and their preparation, can solve problems such as the influence of normal cells, hindering the curative effect of CDT therapy, and harming the normal functions of the human body.

Active Publication Date: 2022-03-08
山东济清科技服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, in tumor cells, H 2 o 2 The content of nano-drugs is small, which is not enough to produce enough hydroxyl radicals, which greatly hinders the curative effect of CDT therapy; at the same time, the introduction of non-tissue-specific nano-medicines will still have a greater impact on normal cells, and will have a negative impact on the normal cells of the human body. Functional damage

Method used

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  • A cell membrane-coated au-fe <sub>3</sub> o <sub>4</sub> Targeted nanomaterials and their preparation methods and applications
  • A cell membrane-coated au-fe <sub>3</sub> o <sub>4</sub> Targeted nanomaterials and their preparation methods and applications
  • A cell membrane-coated au-fe <sub>3</sub> o <sub>4</sub> Targeted nanomaterials and their preparation methods and applications

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preparation example Construction

[0040] The present invention provides the above-mentioned cell membrane coated Au-Fe 3 o 4 A method for preparing a targeted nanomaterial, comprising the following steps:

[0041] (1) Au-Fe 3 o 4 The nanoparticles, tannic acid, phorbol 12-myristate 13-acetate and solvent are shaken and mixed to obtain a mixed solution;

[0042] (2) Mix the cancer cell membrane with the mixed solution, and extrude the porous membrane to obtain the cell membrane-coated Au-Fe 3 o 4 targeted nanomaterials.

[0043] The present invention will Au-Fe 3 o 4 The nanoparticles, the tannic acid, the phorbol 12-myristate 13-acetate and the solvent are shaken and mixed to obtain a mixed liquid. In the present invention, the Au-Fe 3 o 4 The preparation method of nanoparticles preferably comprises the following steps:

[0044] (a) heating and mixing gold nanoparticles, cetyl ammonium chloride, 1-octadecene, oleylamine and organic iron salts under anaerobic conditions to obtain Au-Fe nanoparticles;...

Embodiment 1

[0078] (1) Synthesis of gold nanoparticles

[0079] (1) Under the gentle flow of nitrogen, 0.2g HAuCl 4 ·3H 2 O, 10mL 1,2,3,4-tetrahydronaphthalene and 10mL oleic acid amine were stirred and mixed until the liquid was orange-yellow; the above solution was cooled to 4°C in an ice bath;

[0080] (2) Dissolve 0.5 mmol of borane-tert-butylamine in a mixed solution of 1 mL of 1,2,3,4-tetrahydronaphthalene and 1 mL of oleylamine, dissolve it completely, and then pour it into the mixture obtained in step (1) solution, at this moment, the solution was dark purple, and the reaction continued for 2h;

[0081] (3) After the reaction is complete, add 50 mL of acetone, centrifuge at 10,000 r / min for 8 min, add 20 mL of n-hexane and 50 mL of ethanol to the resulting solid, and centrifuge at 9,000 r / min for 8 min to obtain gold nanoparticles, which are stored in n-hexane at 4°C in spare.

[0082] (2) Synthesis of Au-Fe nanoparticles

[0083] (1) Under argon flow, mix 1 mmol of HDAHCl so...

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Abstract

The invention provides a cell membrane coated Au-Fe 3 o 4 The targeted nanomaterial and its preparation method and application belong to the technical field of targeted nanomaterials. Targeted nanomaterial cancer cell membrane provided by the present invention and Au-Fe wrapped in the cancer cell membrane 3 o 4 Nanoparticles, tannic acid and phorbol 12‑myristate 13‑acetate. PMA can effectively stimulate cells to produce H 2 o 2 and reactive oxygen species 2 ‑ , can suppress the insufficient H in cancer cells 2 o 2 Supplement to supplement raw materials for Fenton reaction; Fe 3 o 4 Can be partially decomposed in the presence of tannic acid to form Fe 2+ and Fe 3+ , Fe 2+ Can catalyze H in cancer cells 2 o 2 Decomposed into hydroxyl radicals, TA can promote Fe 3 o 4 Produced Fe 3+ to Fe 2+ Rapid transformation; at the same time, Au and Fe 3 o 4 It has good photothermal conversion efficiency and can give full play to the therapeutic effect of PTT.

Description

technical field [0001] The invention relates to the technical field of targeted nanomaterials, in particular to a cell membrane-coated Au-Fe 3 o 4 Targeted nanomaterials and their preparation methods and applications. Background technique [0002] For centuries, cancer has been one of the focal issues threatening human health, and many scientific researchers have worked hard to overcome this problem. So far, various treatment methods have been researched and developed, such as chemodynamic therapy (CDT therapy), photodynamic therapy (PDT therapy), photothermal therapy (PTT therapy), etc., among which, the combination of CDT therapy and PTT therapy has produced Good therapeutic effect. CDT treatment mainly utilizes metal and H 2 o 2 The highly active hydroxyl radicals (·OH) produced by the Fenton reaction between them can kill tumor cells. Nano-catalyzed drugs currently available for CDT therapy mainly contain Fe 2+ 、Cu 2 + , Mn 2+ and Co 2+ and other metal ions. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61K47/46A61K41/00A61P35/00B82Y5/00B82Y30/00B82Y40/00A61K31/7024A61K33/242A61K33/26
CPCA61K41/0052A61K9/5068A61K41/00A61K9/0009B82Y5/00B82Y30/00B82Y40/00A61P35/00A61K33/242A61K33/26A61K31/7024A61K31/23A61K2300/00
Inventor 郭英姝曹秀萍
Owner 山东济清科技服务有限公司
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