Immune cell culture method
A culture method and technology of immune cells, applied in the field of immune cell culture, can solve the problems of low culture efficiency, few cells, complicated manual operation process, etc.
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[0040] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0041] Such as figure 1 As shown, the present invention provides a method for culturing immune cells, comprising the steps of:
[0042] Step S1: Preparatory work: culture medium (in an incubator at 37°C) and lymphocyte separation medium were rewarmed (at room temperature).
[0043] Step S1: Sample dilution: Dilute the lower layer of erythrocytes after separation of plasma by adding an equal volume of normal saline, fully mix by pipetting and mixing mechanism,...
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