Preparation method of physalis alkekengi extract and application of physalis alkekengi extract as bulk drug
A technology of brocade lanterns, extracts, applied in the field of medicine
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Embodiment 1
[0030] Step Ⅰ: take 50g brocade lantern medicinal material and cut into pieces, put it into a flask;
[0031] Step Ⅱ: Take 1.5L of 70% ethanol prepared with 95% ethanol and add it to the flask, and carry out the first reflux extraction operation on the shredded brocade lantern medicinal material in the flask, and the 70% ethanol in the flask starts to boil to keep the The 70% ethanol in the flask is boiling state 2h, then extract the first filtrate in the first part of reflux liquid with suction filtration, keep the filter residue in the first part of reflux liquid;
[0032] Step III: Add the filter residue into the flask, and use the 70% ethanol remaining in the flask after the first reflux extraction operation to perform a second reflux extraction on the filter residue in the flask after adding the filter residue Operation, the remaining 70% ethanol in the flask starts to boil and keep the remaining 70% ethanol in the flask in a boiling state for 2h, then extract the second ...
Embodiment 2
[0035] Step Ⅰ: take 45g brocade lantern medicinal material and cut into pieces, put it into a flask;
[0036] Step Ⅱ: Take 1.5L of 70% ethanol prepared by recovering ethanol and add it to the flask, and carry out the first reflux extraction operation on the shredded brocade lantern medicinal material in the flask, and the 70% ethanol in the flask starts to boil to keep the The 70% ethanol in the flask is in boiling state for 2h, then extract the first filtrate in the first part of reflux liquid by suction filtration, and keep the filter residue in the first part of reflux liquid;
[0037] Step III: Add the filter residue into the flask, and use the 70% ethanol remaining in the flask after the first reflux extraction operation to perform a second reflux extraction on the filter residue in the flask after adding the filter residue Operation, the remaining 70% ethanol in the flask starts to boil and keep the remaining 70% ethanol in the flask in a boiling state for 2h, then extra...
Embodiment 3
[0040] The application of brocade lantern extract as a raw material drug as a test substance for inhibiting the release of hyaluronidase
[0041] (1) prepare the acetic acid buffer solution of pH=5 with glacial acetic acid dilution solution and sodium acetate crystallization aqueous solution, stand-by; Prepare respectively the hyaluronidase solution and the concentration that are 0.5g with the prepared acetic acid buffer solution that concentration is 1250U / mL / L sodium hyaluronate solution;
[0042] (2) Prepare brocade lantern extract according to the method of embodiment 1 or embodiment 2, and the prepared brocade lantern extract is formulated into mass concentration respectively 0.10g / L, 0.25g / L, 0.50g / L, 1.00 g / L, 1.50g / L, 2.00g / L, 2.50g / L brocade lantern extract, as the experimental group; the preparation mass concentration is respectively 0.10g / L, 0.25g / L, 0.50g / L, 1.00g / L, 1.50g / L, 2.00g / L, the dipotassium glycyrrhizinate solution of 2.50g / L as positive control group;...
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