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Nucleic acid extraction/detection device and method

A detection device, nucleic acid technology, used in biochemical equipment and methods, enzymology/microbiology devices, biochemical cleaning devices, etc.

Inactive Publication Date: 2021-05-07
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned devices are currently very few in the domestic market, and will bring great help to the next stage of epidemic prevention and control.

Method used

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  • Nucleic acid extraction/detection device and method
  • Nucleic acid extraction/detection device and method
  • Nucleic acid extraction/detection device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] In the customs application scenario, each passenger is issued one of the above-mentioned devices on the plane. Each passenger collects a throat swab sample by himself, uses the back end of the cotton swab to puncture the surface sealing film of the first chamber 1, inserts the cotton swab into the first chamber 1 for nucleic acid lysis, the content of the lysate is 2 mL, and the lysis time is 10min; then press the first chamber 1, the puncture needle 7 at the lower end of the first chamber 1 will pierce the lower sealing film 6, so that the lysate in the first chamber 1 flows downward through the nucleic acid extraction membrane 8, and flows into the waste liquid chamber 9; then, press the second chamber 2 and the third chamber 3 clockwise, the puncture needle pierces the lower side sealing film 6 of the second chamber 2 and the third chamber 3 successively, and the nucleic acid extraction membrane 8 The nucleic acid samples in the second chamber 2 and the third chamber...

Embodiment 2

[0051] In some backward and remote areas, after the throat swab sample collection, use the back end of the cotton swab to puncture the surface sealing film of the first chamber 1, insert the cotton swab into the first chamber 1 for nucleic acid lysis, the content of the lysate is 2mL, the lysis time is 10min; then press the first chamber 1, the puncture needle 7 at the lower end of the first chamber 1 will pierce the lower sealing film 6, so that the lysate in the first chamber 1 flows down through the nucleic acid extraction membrane 8, into the waste liquid chamber 9; then, press the second chamber 2 and the third chamber 3 clockwise, the puncture needle pierces the lower sealing film 6 of the second chamber 2 and the third chamber 3 successively, to The nucleic acid sample on the nucleic acid extraction membrane 8 is cleaned, and the contents of the cleaning solution in the second chamber 2 and the third chamber 3 are respectively 2 mL; finally, the waste liquid chamber 9 is...

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Abstract

The invention provides a disposable portable closed device for nucleic acid extraction, enrichment and amplification detection, aiming at meeting the important demand of rapid portable nucleic acid detection at present. According to the invention, a lysis solution, a cleaning solution, an eluent and a nucleic acid amplification solution are pre-buried in different closed and isolated areas of the device, and then the device is simply rotated and extruded, so that related liquids sequentially pass through a nucleic acid adsorption area, sample lysis, nucleic acid purification and nucleic acid amplification detection can be realized step by step, and the requirements of 'sample feeding-result discharging' in on-site rapid nucleic acid detection are really met. Meanwhile, the device is a disposable closed device, so that the problem of cross contamination of amplification products can be effectively reduced and avoided.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection, and in particular relates to a one-time-use, portable integrated airtight device for nucleic acid extraction, enrichment, amplification and detection. The device integrates the functions of nucleic acid lysis, enrichment extraction and amplification detection, and can perform nucleic acid cleavage, enrichment extraction and amplification detection on various samples including throat swabs on site, realizing "sample in-result out" , which effectively solves the problem that in the current nucleic acid detection process, it relies on biosafety laboratories above level 2 to process samples, but cannot perform instant and rapid detection. Background technique [0002] The novel coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is rapidly breaking out in the world. [0003] With the recovery of the domestic economy and the world ...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N15/10
CPCC12N15/1013C12N15/1017
Inventor 常凌乾王杨徐高连侯建华
Owner BEIHANG UNIV
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