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A probiotic skin care product for reducing skin sensitization and preparation method thereof

A technology for skin sensitization and active probiotics, applied in the field of skin care products, can solve the problems of difficult to determine the consistency of the product effect time, without considering the biological activity of probiotics, etc., to inhibit skin allergies or inflammatory reactions, and promote skin barrier repair. , the preparation method is simple

Active Publication Date: 2022-06-28
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, most probiotic skin care products currently on the market do not take into account the biological activity of probiotics, and there are few products with active anti-allergic functions, and it is difficult to determine the consistency of product effects over time

Method used

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  • A probiotic skin care product for reducing skin sensitization and preparation method thereof
  • A probiotic skin care product for reducing skin sensitization and preparation method thereof
  • A probiotic skin care product for reducing skin sensitization and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1) Active formulation and operational preparation:

[0037] Will 4x10 7 CFU Lactobacillus rhamnosus lyophilized powder and 4×10 7 CFU Lactobacillus delbrueckii lyophilized powder, fully mixed with 20 mL of normal saline, and obtained.

[0038] 2) Cell experiments on dendritic cells and macrophages that induce immune tolerance

[0039]CD14(+) monocytes from peripheral blood of 5 patients with allergic dermatitis were isolated, and interleukin (IL)-4 and granulocyte colony-stimulating factor (GM-CSF) were added to induce monocytes to differentiate into dendritic cells. Interferon (IFN)r and GM-CSF induce monocytes to differentiate into macrophages.

[0040] On the 5th day of in vitro cell culture, the induced dendritic cells (DC) and macrophages (Mφ) were divided into four groups and added LPS (LPS group), Lactobacillus rhamnosus (RAM group), German The mixture of Lactobacillus rhamnosus (DEL group), Lactobacillus rhamnosus and Lactobacillus delbrueckii (MIX group, ob...

Embodiment 2

[0052] Will 4x10 7 CFU Lactobacillus rhamnosus lyophilized powder and 4×10 7 CFU Lactobacillus delbrueckii freeze-dried powder, fully mixed with 20 mL of normal saline, mixed with 40,000 IU of recombinant human epidermal growth factor, shake well to prepare 20 mL of active moisturizing spray liquid, pour the moisturizing spray liquid into a spray bottle use.

Embodiment 3

[0054] The study included 5 normal people aged 18-35 years. The inner skin of the left forearm of the research subjects was divided into 3 square areas with a size of 5 × 5 cm, and the active moisturizing spray described in Example 2 (treatment group A) and Lactobacillus rhamnosus were applied externally. Combine with Lactobacillus delbrueckii physiological saline solution (positive control group B) and physiological saline solution (negative control group C), 3 times a day (each time in the test skin area, use a spray bottle to evenly press and spray 5 cm from the skin surface) After 7 days, the skin transepidermal water loss (TEWL), stratum corneum water content (WCSC) and skin oil (SC) content were detected.

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Abstract

The invention discloses a probiotic skin care product for reducing skin allergy and a preparation method thereof. The probiotic skin care product uses active probiotic lyophilized powder as an active raw material, and the probiotic in the active probiotic lyophilized powder is Lactobacillus rhamnosus and Lactobacillus delbrueckii, the quantity ratio of said Lactobacillus rhamnosus and Lactobacillus delbrueckii is preferably 0.5:1-2:1. The probiotic skin care product can be in the form of an active moisturizing spray, preferably including the following components by mass fraction: 2-10% active probiotic freeze-dried powder, recombinant human epidermal growth factor 2000IU / mL, and the rest being physiological saline. The preparation method of the present invention is simple and easy to popularize, and can be used for whole body skin moisturizing and maintenance including facial care, and can effectively inhibit skin allergy or inflammatory response, reduce skin sensitization, enhance skin resistance to bacterial infection, and promote skin barrier repair.

Description

technical field [0001] The invention belongs to the technical field of skin care products, and in particular relates to a probiotic skin care product with reduced skin sensitization and a preparation method thereof. Background technique [0002] Skin care products are generally skin care cosmetics with beautifying and beauty functions. According to different specific functions and application methods, there are many types and different characteristics. Skin care products can be mainly divided into: cleansing, emollient, whitening, anti-aging, etc. Among them, the emollient skin care products are required to improve the moisturizing effect of the skin surface, so as to maintain the moisturizing property of the skin. There are other types of moisturizing skin care products on the market, such as liquid, gel and mask. [0003] With the rapid development and progress of the cosmetics industry, new types of skin care products are emerging on the market. Among them, many brand m...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/64A61Q19/00A61P29/00A61P37/08
CPCA61K8/99A61K8/64A61Q19/005A61Q19/00A61P29/00A61P37/08A61K2800/84A61K2800/5922A61K2800/10
Inventor 刘昱湛意谭思齐邱月棨
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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