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Single cell control system and method, monoclonal cell line construction method, and single cell droplet generation system and method

A single cell, cell technology, applied in the field of life science and medicine, can solve problems such as high operator proficiency requirements, pollution, and complex equipment

Active Publication Date: 2021-05-04
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantage of the mechanical contact method is that it requires high proficiency of the operator and the equipment is complicated.
Optical tweezers can easily cause irreversible damage to cells, thus affecting the subsequent amplification of genetic material
Magnetic tweezers need to label cells, and this process may also cause pollution. The disadvantage of electrophoresis and other methods is that there are special requirements for the manipulation medium

Method used

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  • Single cell control system and method, monoclonal cell line construction method, and single cell droplet generation system and method
  • Single cell control system and method, monoclonal cell line construction method, and single cell droplet generation system and method
  • Single cell control system and method, monoclonal cell line construction method, and single cell droplet generation system and method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Such as figure 1 As shown, the present application provides a selective single-cell manipulation system based on acoustofluidic tweezers, including:

[0073] The sample buffering device 101 is used for fixing and storing samples; including: for inoculating and culturing samples containing single cells in the sample buffering device;

[0074] The ultra-high frequency bulk acoustic wave resonator 102 is connected with the sample buffer device through a liquid medium, and is used to act on the sample in the sample buffer device to manipulate single cells in the sample; wherein, the ultra-high frequency bulk acoustic wave resonant The frequency generated by device 102 is GHZ.

[0075] A multi-degree-of-freedom moving device 103, which is mechanically connected to the UHF bulk acoustic wave resonator to control its multi-degree-of-freedom movement;

[0076] The PLC controller 104 is used to adjust the spatial position of the ultrahigh frequency bulk acoustic wave resonator...

Embodiment 2

[0096] The present application also provides a selective single-cell manipulation method based on acoustofluidic tweezers, the single-cell manipulation system based on the embodiment, including steps:

[0097] S201, inject the buffer solution into the sample buffer device with cultured cells, so that the target cells have a liquid environment suitable for manipulation; and control the distance between the ultra-high frequency bulk acoustic wave resonator and the cells to about 0-5mm (corresponding to figure 2 a) in; optional, 0-2mm.

[0098] S202, acting on the liquid above the target cell through the acoustic wave action area of ​​the ultra-high frequency bulk acoustic wave resonator, so that the liquid generates a vortex, so that the target cell is stripped from the wall of the sample buffer device under the action of the vortex ( correspond figure 2 in b).

[0099] Among them, by controlling the different powers applied to the UHF BAW resonator and the distance between ...

Embodiment 3

[0104] The present application also provides a method for assembling cell aggregates, which is also based on the selective single-cell manipulation of acoustofluidic tweezers, based on the single-cell manipulation system of the embodiment, including steps:

[0105] S301, inject the buffer solution into the sample buffer device with the target single cell cultured, and control the distance between the UHF bulk acoustic resonator and the target single cell; the range between the UHF bulk acoustic resonator and the target single cell is 0-5mm; The optional range is 0-2mm.

[0106] S302. Act on the liquid above the target single cell through the acoustic wave action area of ​​the ultra-high frequency bulk acoustic wave resonator, so that the liquid generates a vortex, so that the target single cell is removed from the wall of the sample buffering device under the action of the vortex. peel off;

[0107] Among them, by controlling the different powers applied to the UHF bulk acous...

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Abstract

The invention discloses a single cell control system and method based on acoustic fluid tweezers, a monoclonal cell line construction method and a single cell droplet generation method. The single cell control system comprises a sample caching device, an ultrahigh-frequency bulk acoustic wave resonator, a multi-degree-of-freedom motion device and a PLC; the sample caching device is used for fixing and storing different types of samples; the ultrahigh-frequency bulk acoustic wave resonator is connected with the sample caching device through a liquid medium and acts on target cells in the sample caching device; the multi-degree-of-freedom motion device is mechanically connected with the ultrahigh-frequency bulk acoustic wave resonator and controls the ultrahigh-frequency bulk acoustic wave resonator to move; and the PLC is used for adjusting the spatial positions of the ultrahigh-frequency bulk acoustic wave resonator and the cells by controlling the multi-degree-of-freedom motion device and controlling the power and the frequency applied to the ultrahigh-frequency bulk acoustic wave resonator so as to realize stable stripping of adherent single cells and capture of suspension cells, controlling the multi-degree-of-freedom motion device so that the cells move along with the movement of the ultrahigh-frequency bulk acoustic wave resonator, and controlling the release of the captured single cells by controlling the on-off of the power applied to the ultrahigh-frequency bulk acoustic wave resonator.

Description

technical field [0001] The present invention relates to the fields of life science and medicine, and particularly relates to a single cell manipulation system and method based on acoustofluidic tweezers, a method for constructing a monoclonal cell line, and a method for generating single cell droplets. Background technique [0002] The micro-scale manipulation of biological particles is the most basic biotechnology, and it is also the cornerstone technology of cutting-edge research. With the continuous expansion of human cognition, single-cell research continues to lead people to understand the heterogeneity of cell physics and function, which is an important way for biomedical research and clinical diagnosis. Precise single-cell manipulation technology Single-cell manipulation provides the basis for cell analysis and plays a pivotal role in various subsequent processes. With the development of stem cells and tumor cells, more and more attention has been paid to the researc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/00C12N5/00
CPCC12M47/04C12M41/48C12N5/00C12N2509/10
Inventor 段学欣金克杨洋庞慰
Owner TIANJIN UNIV
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