Device for detecting polysaccharide in fermentation liquor based on Raman spectroscopy and method and application thereof

Abstract

The invention provides a device for detecting polysaccharide in fermentation liquor based on Raman spectroscopy and a method and application thereof, and belongs to the technical field of fermentation liquor detection. The device for detecting the polysaccharide in the fermentation liquor based on the Raman spectrum comprises a fermentation tank, a reflector, an optical fiber probe, an optical fiber, an excitation light source, a detector, a signal acquisition module, an A / D conversion module, a single chip microcomputer and a display module, wherein the reflector is located in the fermentation tank, the excitation light source is connected with the optical fiber probe through an optical fiber, the optical fiber probe is connected with the detector through an optical fiber, the detector is further connected with the signal acquisition module, the signal acquisition module is connected with the A / D conversion module, the A / D conversion module is connected with the single-chip microcomputer, and the single-chip microcomputer is connected with the display module. Compared with the traditional polysaccharide detection method, the device provided by the invention does not need purification and separation, simplifies the detection steps, saves the detection time, reduces the polysaccharide detection cost, and has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of fermentation liquid detection, and in particular relates to a device for detecting polysaccharides in fermentation liquid based on Raman spectroscopy, a method and an application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0004] There are a lot of polysaccharides in various ...

AI Technical Summary

Problems solved by technology

[0006] The precision of the HPLC detection device is high, but this method takes too long and the pretreatment is troublesome; the minimum detection limit of the ELISA detection method is low and the sensitivity is high, but the detection steps of this method are cumbersome and require multiple washings; The determination steps of dinitrosalicylic acid method and phenol-sulfuric acid method are very cumbersome

These traditional determination methods cannot reflect the components in the fermentation broth in real time, and the detection results have serious hysteresis

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