Observable chick embryo transferring incubation design

Abstract

The invention discloses an observable transferred chick embryo incubation technology which comprises the steps of incubation device construction and egg transferring incubation. The structure of an incubation device and the specific incubation process are optimized on the basis of an existing egg shell replacement chick embryo culture method, and the incubation device comprises an outer-layer hard container, a middle-layer egg-shaped container and an inner-layer egg shell; bacteriostatic water is contained in the outer-layer hard container, at least one hole is formed in the side face of the outer-layer hard container, and the hole is blocked through a cotton ball; the middle-layer egg-shaped container is a film egg-shaped container; and the inner-layer egg shell is a different-shell egg shell, and an observation opening is formed in the different-shell egg shell. The egg transferring incubation comprises the steps of transferring egg liquid containing chick embryos into the incubation device, constructing a bacteriostatic incubation environment, performing first constant-temperature incubation, ventilating and performing second constant-temperature incubation. The chick embryo incubation process is convenient to observe, meanwhile, the incubation rate is increased, and the hatching health condition is also improved.

Description

technical field [0001] The invention relates to a hatching method, in particular to a hatching design for observable transfer of chicken embryos. Background technique [0002] In the natural state, chicken embryos are hatched in the original eggs. At this time, due to the surrounding of the eggshell, the hatching process can only be observed by distinguishing the transparency of the eggs. It is difficult to clearly observe the hatching changes of the chicken embryos and it is difficult to conduct treatment experiments on the chicken embryos. Transferring chicken embryos to other carriers for incubation and creating an observable window is an idea to overcome the above-mentioned defects. [0003] At present, there are hatching technologies that transfer chicken embryos to plastic wrap, including completely shellless chicken embryo culture methods using glassware, plastic containers, etc. Shell culture method. Among them, there are calcium deficiency and hypoxia problems in ...

AI Technical Summary

Problems solved by technology

Although it is possible to increase the hatching success rate of completely shellless chicken embryo culture by adding calcium lactate as a calcium supplement, supplementing oxygen with the help of ventilation pipes, etc. The utilization mechanism and apparent utilization rate of calcium in calcium lactate are not yet clear, which leads to a high deformity rate of hatched chicken embryos, prone to abnormal bone development, and embryo bodies are smaller than normal hatched chicken embryos

The chicken embryo culture method that replaces the eggshell is to transfer the chicken embryo to the cut duck egg shell, goose egg shell, etc., and then cover it with a plastic wrap. Compared with the former, this method solves the calcium deficiency in the hatching process of the chicken embryo Problem, however, in the prior art replacement shell chicken embryo culture method, before the chicken embryo is transferred to the cut replacement shell, the original egg should be pre-incubated to overcome the difficulty of starting the embryo in the early stage, resulting in low hatching efficiency, And there is still the problem of lack of oxygen and bacterial pollution that is difficult to balance. On the whole, the success rate of hatching is still lower than 50%.

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