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Mild decolorization method of abelmoschus manihot polysaccharide

A technology of hollyhock flowers and hollyhock flowers, which is applied in the field of mild decolorization of polysaccharides from hollyhock flowers, can solve the problems of affecting the activity of polysaccharides, long operating hours, and high costs

Active Publication Date: 2021-04-23
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the cost of activated carbon method and hydrogen peroxide method is relatively low, the operation is cumbersome and the operation time is long, and the decolorization rate is low, the loss of polysaccharide is large, the purity of polysaccharide obtained is low, and the activity of polysaccharide will be affected, which is difficult to meet the market demand.
[0004]Common effective decolorization resins include macroporous adsorption resins, cation exchange resins and anion exchange resins, but after using only resins to adsorb pigments, the column material is difficult to regenerate and the cost is high , not conducive to large-scale use

Method used

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  • Mild decolorization method of abelmoschus manihot polysaccharide
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  • Mild decolorization method of abelmoschus manihot polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065]Mild decolorization method of yellow hambonic flower polysaccharide, including the following steps:

[0066]S1 selected the corolla of the yellow hambonic, pulverized after drying at 60 ° C for 2 hours, 20 mesh screen screen, after sieving the fine powder, get the yellow squid flower, then placed in a dry place;

[0067]S2 weigh 150g after dry, yellow squid, according to the quality than Huamaiki flower powder: Acetone = 1: 8 wash, soak the yellow huni flower crown powder into acetone in 18h, then dry, then according to the mass than Huami flower crown powder: absolute ethanol = 1: 6, soak the yellow gamphi flower into anhydrous ethanol in anhydrous ethanol, take out the yellow hambonic flower powder to ventilate, dry dry, dry, dry, dried, the dry taper, 50 ° C, to obtain the degreasing yellow squid flower crown powder;

[0068]S3 mixed the degreasing yellow squid flower crown powder in accordance with the liquid ratio of 1:20 and the ultrapureated aqueous solution, heated to 60 ° C to...

Embodiment 2

[0103]Change S2 to the quality than Yellow Holly Cape Powder: Acetone = 1: 6, soak the yellow huni flower crown into acetone in acetone, then dry, then according to the mass than Huami flower crown powder: absolute ethanol = 1: 4, put Huamiki flower crown powder Soak in anhydrous ethanol in 12 h;

[0104]At the same time, the evaporation of S3 was concentrated to 2 / 7 of the original volume, and the precipitation concentrate 24h;

[0105]The reduction of low temperature in S5 is concentrated to 1 / 2 of the original volume;

[0106]The crude polysaccharide powder was added to 100 mL of 0.05 mol / L of ethylenediamine tetra acetacetate, and the magnetic force was stirred for 30min, then concentrated to the original volume of 1 / 4;

[0107]The reduced pressure was concentrated to 2 / 7 of the original volume in S7, and the remaining steps and formulations were in Example 1.

Embodiment 3

[0109]Change S2 is more than Yellow Hardi flower crown powder: acetone = 1: 8, soak the yellow huni flower crown powder into acetone in acetone, then dry, then according to the quality than Hua Shiki flower powder: no water ethanol = 1: 5, put Huamiki flower crown powder Soak in anhydrous ethanol in 6 h;

[0110]At the same time, the evaporation of S3 was concentrated to 1 / 3 of the original volume, and the precipitation concentrate 12h;

[0111]S5 is reduced by low temperature to 1 / 4 of the original volume;

[0112]The crude polysaccharide powder was added to 200 mL of sodium 0.6 mol / L of ethylenediamine tetra acetate, magnetic force for 30min;

[0113]The residual steps and formulations were concentrated under S7, and the remaining steps and formulations were in Example 1.

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Abstract

The invention discloses a mild decolorization method of abelmoschus manihot polysaccharide. The method comprises the following steps of S1, preparing abelmoschus manihot corolla powder, S2, preparing degreased abelmoschus manihot corolla powder, S3, preparing a preservation solution, S4, judging whether a protein absorption peak exists or not, S5, preparing crude polysaccharide powder, S6, preparing a polysaccharide concentrated solution, and S7, preparing decolorized abelmoschus manihot polysaccharide powder, and measuring the content of components of the abelmoschus manihot polysaccharide powder; The method has the beneficial effects that the corolla of the abelmoschus manihot is ground and crushed, washed and degreased by acetone and absolute ethyl alcohol, repeatedly extracted for multiple times, subjected to calcium chloride and pH control and dialyzed by a dialysis bag, so that protein is precipitated and separated from a polysaccharide solution, and finally, the disodium ethylene diamine tetraacetate and the exchange resin are combined, so that the structure of the polysaccharide is not damaged, the activity is not lost, the decolorization rate is improved, the loss rate of the polysaccharide is relatively low, and the utilization efficiency of the device is improved.

Description

Technical field[0001]The present invention relates to the field of pharmaceutical component extraction, and is specifically a temperature and decolorization method of yellow squid.Background technique[0002]Huang Shiki is the Kamikikikiki, which is a one or more born and standing plants, alias, Nikra, Cotton Sunflower, Wild Furg, Chicken, and China, mainly distributed in Yunnan in my country. Guizhou, Sichuan, Hubei, Guangdong, Guangxi and Taiwan and other provinces; Huang Yuxi Corolla is a traditional Chinese herbal medicine, usually used as many different purposes such as herbs, tea and health care, which has high health care value, such as beauty Yang Yan, increase appetite, assisted treatment of nephropathy, treat inflammation.[0003]As a traditional Chinese herbal medicinal material, Huang Xiaihua is widely distributed, diverse and low-cost, polysaccharides are the main pharmacological active substances in Huaqua, which has a variety of biological functions such as anti-tumor, an...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 何钢朱俊豪张彩梅梁立刘嵬颜军刘坤平孙晓华
Owner CHENGDU UNIV
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